Size | Price | Stock | Qty |
---|---|---|---|
1mg |
|
||
Other Sizes |
|
Targets |
Ex: 530 nm; Em 557 nm
|
---|---|
ln Vitro |
HKOCl-4 (10 μM) exhibits a greater degree of florescence with an increase in hypochlorous acid concentration[1]. The fluorescence intensity of HKOCl-4 is >20 times higher than that of other analytes (1 equiv of OH and ONOO as well as 10 equiv of O2, NO, 1O2, ROO, TBHP, and H2O2), and it responds extremely quickly to 1 equiv of HOCl (hit a plateau within 2 min)[2]. After being evaluated on RAW264.7 macrophages up to 50 μM, HKOCl-4 was found to be non-toxic in living cells[2].
|
References |
[1]. Dan Yang, et al. Diarylether-based fluorogenic probes for detection of hypochlorous acid or hydroxyl radical. Patent US20160312033.
[2]. Xiaoyu Bai, et al. HKOCl-4: a rhodol-based yellow fluorescent probe for the detection of hypochlorous acid in living cells and tissues. |
Molecular Formula |
C38H35CL2NO7
|
---|---|
Molecular Weight |
688.59
|
CAS # |
2031170-85-9
|
Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
|
SMILES |
N1(CCCC(OCC)=O)C2=C(C=C3C4(C5=C(C=CC=C5)C(=O)O4)C4=CC=C(OC5=CC(Cl)=C(O)C(Cl)=C5)C=C4OC3=C2)C(C)CC1(C)C
|
HS Tariff Code |
2934.99.9001
|
Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
Solubility (In Vitro) |
DMSO: 200 mg/mL (290.45 mM)
|
---|---|
Solubility (In Vivo) |
Solubility in Formulation 1: 5 mg/mL (7.26 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.4522 mL | 7.2612 mL | 14.5224 mL | |
5 mM | 0.2904 mL | 1.4522 mL | 2.9045 mL | |
10 mM | 0.1452 mL | 0.7261 mL | 1.4522 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.