Size | Price | |
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100mg | ||
250mg | ||
500mg | ||
Other Sizes |
ln Vitro |
Guidelines[2] (The protocol listed below is our suggested one; it should be adjusted to meet your unique requirements as it just serves as a guide). Cell Labeling: 1. Proceed with the regular protocol for incubating the cells. 2. In 37°C Hanks Balanced Salt Solution (HBSS) with 1 μM Fura Red, AM (unless otherwise noted) and 0.01% Pluronic F127, suspend cells at 1x107 cells/mL. Incubate for 30 minutes in a 37°C water bath. 3. Re-suspend cells to 1x107 cells/mL in HEPES Buffered Saline Solution using viability dye, SYTOX Green, after washing them in HEPES Buffered Saline Solution (HBSS with 1 mM CaCl2, 0.5 mM MgCl2, 0.1% BSA, and 10 mM HEPES). 4. Cells should equilibrate in a water bath at 37°C for at least ten minutes. 5. Use a flow cytometer, fluorescence microscopy, or fluorescence microplate reader to analyze the sample. Fura Red is measured using ratiometric analysis using two lasers: the green laser (532 nm) and the violet laser (406 nm). Two distinct filter sets are used to detect emission: the Violet laser (630LP and 660/20 BP) is used to monitor increases in emission, while the Green laser (685LP and 710/50 BP) is used to detect decreases in emission.
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References |
[1]. Floto RA, et al. IgG-induced Ca2+ oscillations in differentiated U937 cells; a study using laser scanning confocal microscopy and co-loaded fluo-3 and fura-red fluorescent probes. Cell Calcium. 1995 Nov;18(5):377-89.
[2]. Wendt ER, et al. Ratiometric analysis of fura red by flow cytometry: a technique for monitoring intracellular calcium flux in primary cell subsets. PLoS One. 2015 Apr 2;10(3):e0119532. |
Molecular Formula |
C41H44N4O20S
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Molecular Weight |
944.87
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CAS # |
149732-62-7
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
CC(OCOC(CN(C1C=CC(C)=CC=1OCCOC1C=C2C=C(OC2=CC=1N(CC(OCOC(=O)C)=O)CC(OCOC(=O)C)=O)/C=C1\NC(=S)NC\1=O)CC(OCOC(=O)C)=O)=O)=O
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.0583 mL | 5.2917 mL | 10.5835 mL | |
5 mM | 0.2117 mL | 1.0583 mL | 2.1167 mL | |
10 mM | 0.1058 mL | 0.5292 mL | 1.0583 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.