Size | Price | |
---|---|---|
100mg | ||
250mg | ||
500mg | ||
Other Sizes |
ln Vitro |
First general protocol. DAPI dilactate working solution preparation 1.1: provide a stock solution by dissolving 5 mg of DAPI dilactate in 1 mL of ddH2O. This will provide a stock solution that is 5 mg/mL (DAPI, dilactate 10.9 mM). Note: It is advised to keep the stock solution frozen and to store it in the dark at -20°C or -80°C. 1.2 Making the DAPI dilactate working solution: Dilute the original solution in PBS to a concentration of 1 μg/mL (1:5000). Work solutions should be kept at 4 °C. Note: Please change the DAPI dilactate working solution's concentration as necessary. 2. Suspension cells (96-well plate) 2.1 Cell staining a. Discard the supernatant after centrifuging at 1000g for three to five minutes at 4°C. Use PBS to wash twice, for five minutes each time. The density of cells is 1×106/mL. b. Incubate for 3–10 minutes at room temperature after adding 1 mL of working solution. C. After centrifuging for three to four minutes at 400 g and 4°C, remove the supernatant. d. Use PBS to wash twice, for five minutes each time. e. Re-suspend cells in PBS or serum-free culture media. observation using flow cytometry or fluorescence microscopy. 2.2 Cells that adhere a. On sterile coverslips, cultivate adherent cells. b. Aspirate extra culture medium after removing the coverslip from the medium. C. To fully submerge the cells, add 100 μL of working solution, give them a little shake, and let them sit at room temperature for three to ten minutes. d. Use culture media for five minutes each time you wash, twice. observation using flow cytometry or fluorescence microscopy. Precautions: The stock solution can be aliquoted and kept at ≤-20°C for long-term storage. The solution can be kept for a brief period of time at 2–6°C in a dark place. DAPI solutions are reliable for at least six months when managed correctly.
|
---|---|
References |
[1]. Kapuscinski J. DAPI: a DNA-specific fluorescent probe. Biotech Histochem. 1995 Sep;70(5):220-33.
[2]. Nazaroff CD, et al. Assessment of Lung Eosinophils In Situ Using Immunohistological Staining. Methods Mol Biol. 2021;2223:237-266. |
Molecular Formula |
C22H27N5O6
|
---|---|
Molecular Weight |
457.48
|
CAS # |
28718-91-4
|
Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
|
SMILES |
CC(C(=O)O)O.NC(C1C=CC(C2=CC3C=CC(=CC=3N2)C(=N)N)=CC=1)=N
|
HS Tariff Code |
2934.99.9001
|
Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
---|---|
Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.1859 mL | 10.9294 mL | 21.8589 mL | |
5 mM | 0.4372 mL | 2.1859 mL | 4.3718 mL | |
10 mM | 0.2186 mL | 1.0929 mL | 2.1859 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.