Size | Price | |
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100mg | ||
250mg | ||
500mg | ||
Other Sizes |
ln Vitro |
Guidelines (This is our suggested protocol; it should be adjusted based on your unique requirements as it just offers as a guideline). Fluorescent internal protein labeling: A. samples of protein ready: 1. Protein samples that are going to be examined are first spiked with 0.1% of the ALIS647 (ALIS internal standard) total protein load. 2. 2-DE is used to separate the protein sample in the dark. B. To make sulforhodamine G pure: 1. 10 mg of 60% pure sulforhodamine G was diluted in 100 milliliters of 1% v/v acetic acid for RP chromatography purification. 2. Gather the pool whose absorbance peaks at 528 nm, lyophilize it till it becomes completely dry, and then store it at 47°C as a dry powder. C. Holding: 1. To avoid the stains photobleaching, staining was done in polypropylene staining dishes covered with aluminum foil. 2. Proteins with an average molecular weight of 50 kDa are stained with sulforhodamine G over night in 35% methanol using a four-fold molar excess of dye. 3. After 4 ×15 minutes of washing in 35% methanol and 2 ×15 minutes of water equilibration. 4. Using separate channels, a laser scanner visualizes total protein and ALIS. 5. Protein spots observed with total protein stain (λex=532 nm) and ALIS (λex=633 nm) are independently quantified by statistical analysis and 2-DE software.
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References |
[1]. Asa M Wheelock, et al. Use of a fluorescent internal protein standard to achieve quantitative two-dimensional gel electrophoresis. Proteomics. 2006 Mar;6(5):1385-98.
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Molecular Formula |
C25H25N2NA2O7S2+
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Molecular Weight |
575.58
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CAS # |
5873-16-5
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
[Na+].CCNC1C(C)=CC2C(C3C=CC(S([O-])(=O)=O)=CC=3S(=O)(O)=O)=C3C=C(C)/C(=N/CC)/C=C3OC=2C=1
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.7374 mL | 8.6869 mL | 17.3738 mL | |
5 mM | 0.3475 mL | 1.7374 mL | 3.4748 mL | |
10 mM | 0.1737 mL | 0.8687 mL | 1.7374 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.