Method of usage 1. The Golgi working solution is prepared as follows: 1.1 Stock solution preparation: make a 5 mM stock solution using DMSO. Note: To prevent recurrent freeze-thaw cycles, it is advised to store the stock solution at -20°C or -80°C in the dark. 1.2 Working solution preparation: Make 1–10 μM Golgi working solution using hot PBS or culture medium solution. Note: Please get ready for the current use of Golgi 1-43 working fluid by adjusting its concentration according on the actual circumstances. 2. Suspension cell staining (2.1): Gather cells at 1,000 g, 4°C, centrifuge for three to five minutes, and remove the supernatant. Include PBS and wash for five minutes each time, twice. The density of cells is 1×106/mL. 2.2 After adding 1 mL of Golgi working solution, incubate for 20 to 30 minutes at room temperature. 2.3 Centrifuge for 3–4 minutes at 400 g and 4°C; discard supernatant. 2.4 After adding PBS, wash the cells twice for five minutes each. 2.5 Re-suspend the cells in 1 milliliter of PBS or serum-free media, and use a flow cytometer or fluorescence microscope to observe. 3. Adherent cell staining (3.1): Grow adherent cells on sterile coverslips. 3.2 Aspirate extra culture medium after removing the coverslip from the medium. 3.3 Add 100 μL of dye working solution, give the cells a gentle shake to cover them completely, and then let them sit for 20 to 30 minutes. 3.4 Remove the dye working solution, rinse twice in culture media for five minutes each time, and use a flow cytometer or fluorescence microscope to observe. Storage conditions: -20°C for a year, with protection from light. Precautions: 1. The storage solution should be kept out of direct sunlight, at a temperature of -20°C or -80°C, and not frozen and thawed too frequently. 2. Please modify the Golgi 1-43 working solution's concentration and incubation period based on the current circumstances. 3. Alternately, if the results are poor, wash the cells with a suitable volume of Hanks balanced salt solution after removing the cell growth medium. 4. This product may not be used for clinical diagnosis or treatment, nor may it be included into food or medication. It is intended solely for professional use in scientific study. 5. Please wear disposable gloves and a lab coat for your health and safety.

[1]. Alamudi SH, et al. Development of background-free tame fluorescent probes for intracellular live cell imaging. Nat Commun. 2016 Jun 20;7:11964.

C39H50BF2N3O4S

705.70

571186-05-5

Typically exists as solids (or liquids in special cases) at room temperature

[F-][B+3]1(N2=C(C3SC=CC=3)C=CC2=CC2=CC=C(C3C=CC(OCC(=O)NC(CO)C(O)C=CCCCCCCCCCCCCC)=CC=3)[N-]12)[F-]

2934.99.9001

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples

Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.

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Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)
*Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution.
Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline)
Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil)
Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals).

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Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)]
*Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.
Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline)
Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline)
Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline)
Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline
Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil)
Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline)

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Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium)
Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose
Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals).

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Oral Formulation 3: Dissolved in PEG400
Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose
Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose
Oral Formulation 6: Mixing with food powders

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Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently.

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 (Please use freshly prepared in vivo formulations for optimal results.)