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100mg | ||
250mg | ||
500mg | ||
Other Sizes |
ln Vitro |
Guidelines (This is our suggested protocol; it should be adjusted based on your unique requirements as it just offers as a guideline). 1. Arrange 1×106 cells of every kind of cell on a different 35 mm plate with a glass bottom. 2. After a full day, give the cells a 2-hour incubation period using a 10 µM Cypate Phenol Red-Free Medium solution. 3. Following therapy, cells are collected for spectroscopic and/or LC-MS analysis, or they are fixed with 4% paraformaldehyde for imaging and three PBS washes. 4. Confocal microscopy of research with in vitro cell culture. Cypate has an excitation wavelength of 647 nm[1].
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ln Vivo |
The maximum hepatic accumulation occurs when cipate (10 nmol; 100 µL); intravenously administered every 24 hours; for 6 days[1]. In tumors of Balb/c mice containing 4T1 cells, the fluorescence signal of Cypate (5 mg/kg; i.v.) was faint and quickly deteriorated, presumably because free Cypate was quickly removed from the body. The liver and kidney showed stronger fluorescence in the free cypate group. The IVIS Lumina imaging system (Ex-745 nm; Em-800 nm) was used to assess the Cypate fluorescence signal[2].
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Animal Protocol |
Animal/Disease Models: Foxn1nu/Foxn1nu nude 2.5-month-old female mice with breast cancer cells (MDA-MB-231 Luc2)[1]
Doses: 10 nmol in 100 µL PBS Route of Administration: IV; every 24 hrs (hours) for 6 days Experimental Results: The accumulation alone in the tumor was negligible for 24 hrs (hours) after the injection. The liver had the highest accumulation at all time points. |
References |
[1]. Mona Doshi, et al. Cypate and Cypate-Glucosamine as Near-Infrared Fluorescent Probes for In Vivo Tumor Imaging. Mol Pharmacol. 2019 May;95(5):475-489.
[2]. Yuanyuan Li, et al. Light-Decomposable Polymeric Micelles with Hypoxia-Enhanced Phototherapeutic Efficacy for Combating Metastatic Breast Cancer. Pharmaceutics. 2022 Jan 21;14(2):253. |
Molecular Formula |
C41H40N2O4
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Molecular Weight |
624.77
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CAS # |
95837-47-1
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Related CAS # |
Cypate hydrochloride
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
O=C(CC[N+]1=C(C=CC=CC=CC=C2C(C)(C)C3C(=CC=C4C=3C=CC=C4)N2CCC(O)=O)C(C)(C)C2C1=CC=C1C=2C=CC=C1)[O-]
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.6006 mL | 8.0029 mL | 16.0059 mL | |
5 mM | 0.3201 mL | 1.6006 mL | 3.2012 mL | |
10 mM | 0.1601 mL | 0.8003 mL | 1.6006 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.