| Size | Price | |
|---|---|---|
| 100mg | ||
| 250mg | ||
| 500mg | ||
| Other Sizes |
| ln Vitro |
1. Methods for creating the Fluo-3FF AM working solution and stock solution: Dilute the Fluo-3FF AM solution to make a 1 mM stock solution by adding 0.025% (w/v) Pluronic F-127/dimethyl sulfoxide solution to it. It is suggested that Fluo-3FF AM storage solution be aliquoted and kept in the dark at -20°C or -80°C. 1.2 Making the working solution: Make a 5 μM Fluo-3 AM working solution using the equilibrium buffer solution. Note: Before using Fluo-3FF AM working fluid, please adjust its concentration based on the current circumstances. 2. Cell staining 2.1 Use sterile coverslips to cultivate adherent cells. 2.2 Take off the coverslip from the culture media and use an aspirator to remove any extra. 2.3 Cover the cells completely with 100 μL of the dye working solution, shake gently, and let it sit for 60 minutes. 2.4 Aspirate the dye working solution, then spend 60 minutes washing in PSS at 4°C.
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| References |
[1]. Gordienko DV, et al. Regulation of muscarinic cationic current in myocytes from guinea-pig ileum by intracellular Ca2+ release: a central role of inositol 1,4,5-trisphosphate receptors. Cell Calcium. 2004 Nov;36(5):367-86.
[2]. Young RC, et al. Focal sarcoplasmic reticulum calcium stores and diffuse inositol 1,4,5-trisphosphate and ryanodine receptors in human myometrium. Cell Calcium. 1999 Jul-Aug;26(1-2):69-75. |
| Molecular Formula |
C50H46CL2F2N2O23
|
|---|---|
| Molecular Weight |
1151.80
|
| CAS # |
348079-13-0
|
| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
|
| SMILES |
ClC1C(=CC2=C(C=1)C(=C1C=C(C(C=C1O2)=O)Cl)C1C=CC(=C(C=1)OCCOC1C(=C(C=CC=1N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)F)F)N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)OCOC(C)=O
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.8682 mL | 4.3410 mL | 8.6821 mL | |
| 5 mM | 0.1736 mL | 0.8682 mL | 1.7364 mL | |
| 10 mM | 0.0868 mL | 0.4341 mL | 0.8682 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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