| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
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| Targets |
aPKC-ζ 5 μM (IC50)
|
|---|---|
| ln Vitro |
At 20 μM, ζ-Stat (0.1–20 µM) only exhibits 13% inhibition on PKC–ζ; but, at 5 μM, it exhibits a strong 51% inhibition on PKC–ζ[1]. As the concentrations of ζ-Stat (0.1-10 µM; 3 d) increase, so does the cell proliferation of SK-MEL-2 and MeWo. The combination of 5-FU and ζ-Stat (7 or 10 µM; 24-72 h) can reduce the viability of LoVo CRC cells by about 75%[2]. ζ-Stat (5 µM; 3 d) significantly reduces the levels of Bcl-2, PARP, and phosphorylated total PKC-ζ in SK-MEL-2 and MeWo cells while increasing the levels of caspase-3 and cleaved PARP[1]. On MEL-F-NEO, SK-MEL-2, and MeWo cells, ζ-Stat (5 µM; 1–10 h) does not exhibit appreciable cytotoxicity[1].
|
| Cell Assay |
Cell Proliferation Assay[1]
Cell Types: MEL-F-NEO, SK-MEL-2 and MeWo cells Tested Concentrations: 0.1, 0.5, 1, 2.5, 5, 7.5, 10 µM Incubation Duration: 3 days Experimental Results: diminished proliferation by 47.7% for 5 µM in SK-MEL-2 cells and by 50.6% for 5 µM in MeWo cells. demonstrated significant inhibitions on MEL-F-NEO cells 19.3% (P ≤ 0.05) at 10 μM. Western Blot Analysis[1] Cell Types: SK-MEL-2 and MeWo cells Tested Concentrations: 5 µM Incubation Duration: 3 days Experimental Results: diminished phosphorylated and total PKC-ζ levels. |
| References |
|
| Molecular Formula |
C10H5O10S3-3.3[NA+]
|
|---|---|
| Molecular Weight |
450.3051
|
| Exact Mass |
449.873
|
| CAS # |
31894-34-5
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| PubChem CID |
90471481
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| Appearance |
Light yellow to light brown solid powder
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
10
|
| Rotatable Bond Count |
0
|
| Heavy Atom Count |
26
|
| Complexity |
711
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
[Na+].[Na+].[Na+].O=S(C1=CC(O)=C2C(S([O-])(=O)=O)=CC(=CC2=C1)S([O-])(=O)=O)(=O)[O-]
|
| InChi Key |
VQXXRDCDIFAODW-UHFFFAOYSA-K
|
| InChi Code |
InChI=1S/C10H8O10S3.3Na/c11-8-3-6(21(12,13)14)1-5-2-7(22(15,16)17)4-9(10(5)8)23(18,19)20;;;/h1-4,11H,(H,12,13,14)(H,15,16,17)(H,18,19,20);;;/q;3*+1/p-3
|
| Chemical Name |
trisodium;8-hydroxynaphthalene-1,3,6-trisulfonate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O: 12.5 mg/mL (27.76 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.63 mg/mL (5.84 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2207 mL | 11.1035 mL | 22.2069 mL | |
| 5 mM | 0.4441 mL | 2.2207 mL | 4.4414 mL | |
| 10 mM | 0.2221 mL | 1.1103 mL | 2.2207 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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