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Other Sizes |
ln Vitro |
173 g of 1,4-piperazinediethanesulfonic acid are dissolved in 1 L of deionized water to create the pH PIPES buffer. Pellets of sodium hydroxide are added to the PIPES buffer to bring its pH down to 6.8. Lipid loss and artifacts caused by glutaraldehyde fixation can be avoided by using PIPES buffer [1].
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References |
[1]. Jason Moggridge, et al. Sensitive Detection of Immunoglobulin G Stability Using in Real-Time Isothermal Differential Scanning Fluorimetry: Determinants of Protein Stability for Antibody-Based Therapeutics. Technol Cancer Res Treat. 2017 Dec;16(6):997-1005.
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Molecular Formula |
C8H18N2O6S2
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Molecular Weight |
302.37
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CAS # |
5625-37-6
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Related CAS # |
PIPES disodium;76836-02-7;PIPES-d18;352534-95-3
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
S(C([H])([H])C([H])([H])N1C([H])([H])C([H])([H])N(C([H])([H])C([H])([H])S(=O)(=O)O[H])C([H])([H])C1([H])[H])(=O)(=O)O[H]
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
H2O: 5 mg/mL (16.54 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: 2 mg/mL (6.61 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
 (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.3072 mL | 16.5360 mL | 33.0721 mL | |
5 mM | 0.6614 mL | 3.3072 mL | 6.6144 mL | |
10 mM | 0.3307 mL | 1.6536 mL | 3.3072 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.