| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| Other Sizes |
|
| Targets |
IC50: 11.56 μM (IL6/STAT3 signaling pathway), 3.32 μM (MDA-MB-231), 4.72 μM (H4), 3.14 μM (HepG2)[1]
|
|---|---|
| ln Vitro |
The STAT3, STAT1, and NF-κB signaling pathways are inhibited by angoline (0-100 μM; 1 h), with IC50 values of 11.56, >100, and >100 μM, respectively [1]. The effects of angoline (0-100 μM; 2 h) on STAT3 phosphorylation [1]. Angoline (0-100 μM; 72 h) suppresses the proliferation of HepG2, H4, and MDA-MB-231 cells [1].
|
| Cell Assay |
Western Blot Analysis[1]
Cell Types: HepG2/STAT3 cell line Tested Tested Concentrations: 0, 1, 10, 30 and 100 μM Incubation Duration: 2 hrs (hours) Experimental Results: Inhibited IL-6-induced phosphorylation of STAT3 in HepG2/STAT3 cells. Cell Proliferation Assay[1] Cell Types: MDA-MB-231, H4 and HepG2 cell lines Tested Tested Concentrations: 0-100 μM Incubation Duration: 72 hrs (hours) Experimental Results: Inhibited proliferation of MDA-MB-231, H4 and HepG2 cells with IC50 values of 3.32, 4.72 and 3.14 μM, respectively. |
| References | |
| Additional Infomation |
Angoline is a benzophenanthridine alkaloid.
Angoline has been reported in Chelidonium majus, Macleaya cordata, and other organisms with data available. |
| Molecular Formula |
C22H21NO5
|
|---|---|
| Molecular Weight |
379.41
|
| Exact Mass |
379.142
|
| CAS # |
21080-31-9
|
| Related CAS # |
Angoline hydrochloride;1071676-04-4
|
| PubChem CID |
189060
|
| Appearance |
White to off-white solid powder
|
| Density |
1.36g/cm3
|
| Boiling Point |
555.5ºC at 760 mmHg
|
| Flash Point |
164.1ºC
|
| Vapour Pressure |
2.23E-12mmHg at 25°C
|
| Index of Refraction |
1.678
|
| LogP |
4.412
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
28
|
| Complexity |
561
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
LVWAKZBZWYHYCJ-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C22H21NO5/c1-23-20-14(6-5-12-9-17-18(10-15(12)20)28-11-27-17)13-7-8-16(24-2)21(25-3)19(13)22(23)26-4/h5-10,22H,11H2,1-4H3
|
| Chemical Name |
1,2,13-trimethoxy-12-methyl-13H-[1,3]benzodioxolo[5,6-c]phenanthridine
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 20.83 mg/mL (54.90 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (6.59 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (6.59 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6357 mL | 13.1784 mL | 26.3567 mL | |
| 5 mM | 0.5271 mL | 2.6357 mL | 5.2713 mL | |
| 10 mM | 0.2636 mL | 1.3178 mL | 2.6357 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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