Size | Price | Stock | Qty |
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1mg |
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5mg |
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10mg |
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Other Sizes |
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Targets |
PI3Kα 1.8 nM (IC50)
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ln Vitro |
Compound 10, PI3Kα-IN-4, inhibits PI3Kα, β, δ, and γ in kinase tests, having IC50s of 1.8, 271.0, 13.9, and 13.8 nM, respectively[1]. In cell-based experiments, PI3Kα-IN-4 inhibits PI3Kα, β, δ, and γ with IC50s of 12.1,1393, 183, and >10000 nM, respectively[1].
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ln Vivo |
The most effective chemical, PI3Kα-IN-4 (30 mg/kg; po once daily for 21 days), can decrease tumor growth in mice by 73.0%[1]. In mice, dose-dependently, PI3Kα-IN-4 (15–40 mg/kg; po once daily for 30 days) inhibits tumor growth by 62.5% (15 mg/kg), 86.0% (30 mg/kg), and 90.7% (40 mg/kg), respectively[1]. In a dose- and time-dependent manner, PI3Kα-IN-4 (15–40 mg/kg; po once daily; 1-4 h) suppresses the phosphorylation of Akt in vivo[1]. After oral treatment (mouse 10, rat 3 mg/kg), PI3Kα-IN-4 has a high Cmax (mouse 22167, rat 2327 nM) and good bioavailability (mouse 59.4%, rat 46.9%)[1]. Following intravenous administration (mouse 1, rat 1 mg/kg), PI3Kα-IN-4 exhibits t1/2 (mouse 0.99, rat 1.22 h) and low plasma clearance (mouse 4.16, rat 5.28 mL/min/kg)[1].
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Animal Protocol |
Animal/Disease Models: BT-474 subcutaneous (sc) xenograft mice[1]
Doses: 30 mg/kg Route of Administration: Po one time/day for 21 days Experimental Results: Inhibited tumor growth by 73.0% and could be tolerated. |
References |
[1]. Dong J, et, al. Discovery of 3-Quinazolin-4(3 H)-on-3-yl-2, N-dimethylpropanamides as Orally Active and Selective PI3Kα Inhibitors. ACS Med Chem Lett. 2020 Jun 10; 11(7): 1463-1469.
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Molecular Formula |
C25H23CLFN5O5S
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Molecular Weight |
560.00
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CAS # |
2322293-83-2
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
C1=CC(=C(C=C1F)Cl)S(NC1=CC(C2=CC=C3C(=C2)C(N(C=N3)C[C@H](C(NC)=O)C)=O)=CN=C1OC)(=O)=O
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO: 100 mg/mL (178.57 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (4.46 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (4.46 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (4.46 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.7857 mL | 8.9286 mL | 17.8571 mL | |
5 mM | 0.3571 mL | 1.7857 mL | 3.5714 mL | |
10 mM | 0.1786 mL | 0.8929 mL | 1.7857 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.