Size | Price | Stock | Qty |
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5mg |
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10mg |
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50mg |
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Other Sizes |
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Targets |
5-HT1A Receptor 5-HT1B Receptor 5-HT1D Receptor 5-HT5 Receptor 5-HT7 Receptor
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ln Vitro |
[3H] 5-Carboxamidotryptamine (5-CT) has low non-specific binding and high affinity (Kd = 0.38 nM) for 5-HT1D sites in the bovine substantia nigra. Its binding is also fast, reversible, and saturable[2]. [3H]-5-Carboxamidotryptamine (5-CT) tagged a similar number of binding sites (403 and 362 fmol/mg protein, respectively) in the bovine substantia nigra, and binding is guanine nucleotide-sensitive[2].
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ln Vivo |
In anesthetized Wistar rats (serotonin depletion and treated with 20 mg/kg corticosterone), topical 5-carboxamidotryptamine (0.01-1000 μM) to the exposed dura mater encephala causes decreases in diastolic blood pressure, variable changes in meningeal blood flow, and increases in conductance (i.e. dilatation) in the middle meningeal artery[1].
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References |
[1]. E Martínez-García, et al. 5-HT7 receptor-mediated meningeal dilatation induced by 5-carboxamidotryptamine in rats is not altered by 5-HT depletion and chronic corticosterone treatment. Proc West Pharmacol Soc. 2011;54:57-61.
[2]. H P Nowak, et al. [3H]-5-carboxamidotryptamine labels 5-HT1D binding sites in bovine substantia nigra. Br J Pharmacol. 1993 Aug;109(4):1206-11. [3]. J Yamada, et al. Effects of the non-selective 5-HT receptor agonist, 5-carboxamidotryptamine, on plasma glucose levels in rats. Eur J Pharmacol. 1998 Oct 16;359(1):81-6. |
Molecular Formula |
C15H17N3O5
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Molecular Weight |
319.31
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CAS # |
74885-72-6
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Related CAS # |
5-Carboxamidotryptamine;74885-09-9
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
NC(C1=CC=2C(CCN)=CNC2C=C1)=O.O=C(O)/C=C\C(O)=O
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO: 50 mg/mL (156.59 mM)
H2O: 25 mg/mL (78.29 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.83 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.1318 mL | 15.6588 mL | 31.3175 mL | |
5 mM | 0.6264 mL | 3.1318 mL | 6.2635 mL | |
10 mM | 0.3132 mL | 1.5659 mL | 3.1318 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.