Size | Price | Stock | Qty |
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1mg |
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5mg |
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10mg |
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Other Sizes |
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ln Vitro |
In cells containing the circadian reporter Bmal1-dLuc as well as the other circadian reporter Per2-dLuc, KL201 results in a dose-dependent prolongation of the circadian period. KL201 significantly reduces Per2-dLuc reporter intensity compared to Bmal1-dLuc, all while maintaining cellular viability[1]. KL201 binds to CRY1 in an overlap with FBXL3, a ubiquitin ligase complex subunit, and FBXL3 knockdown attenuates KL201's effect[1]. In mouse lung primary explants, KL201 prolonged the circadian period and reduced the intensity of the Per2::Luc knockin reporter[1].
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References |
[1]. Simon Miller, et al. An Isoform-Selective Modulator of Cryptochrome 1 Regulates Circadian Rhythms in Mammals. Cell Chem Biol. 2020 Sep 17;27(9):1192-1198.e5.
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Molecular Formula |
C17H14BRN3OS
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Molecular Weight |
388.28
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CAS # |
302939-48-6
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
BrC1C=CC=CC=1C(NC1=C2C(=NC=N1)SC1CCCCC=12)=O
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO: 250 mg/mL (643.87 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.36 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.5755 mL | 12.8773 mL | 25.7546 mL | |
5 mM | 0.5151 mL | 2.5755 mL | 5.1509 mL | |
10 mM | 0.2575 mL | 1.2877 mL | 2.5755 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.