Size | Price | Stock | Qty |
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1mg |
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5mg |
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10mg |
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Other Sizes |
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Targets |
PDE4
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ln Vitro |
In MDCK cells, MR-L2 (0.3-10 μM; 1 h) effectively reduces the development of cysts and rise of cAMP[1].
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Cell Assay |
Cell Viability Assay[1]
Cell Types: Madin-Darby Canine Kidney (MDCK) cell line Tested Concentrations: 0.3, 1 , 3 and 10 μM Incubation Duration: 1 hour Experimental Results: Suppressed cAMP elevation but not enhanced cAMP excretion in MDCK cells. Suppressed cysts formation in MDCK cells with an EC50 value of 1.2 µM. Suppressed the number of cysts formation in 3D culture for both unstimulated and vasopressin-treated culture condition while demonstrated no effect on cell viability. |
References |
[1]. Omar F, et al. Small-molecule allosteric activators of PDE4 long form cyclic AMP phosphodiesterases. Proc Natl Acad Sci U S A. 2019 Jul 2;116(27):13320-13329.
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Molecular Formula |
C19H16CL3FN4O
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Molecular Weight |
441.71
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CAS # |
2374703-19-0
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
ClC1C=C(C=C(C=1)CNC(CN1C(CC)=NC(C2C=CC(=C(C=2)F)Cl)=N1)=O)Cl
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO: 83.33 mg/mL (188.65 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (4.71 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.2639 mL | 11.3196 mL | 22.6393 mL | |
5 mM | 0.4528 mL | 2.2639 mL | 4.5279 mL | |
10 mM | 0.2264 mL | 1.1320 mL | 2.2639 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.