| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
|
||
| Other Sizes |
|
| Targets |
Microbial Metabolite
|
|---|---|
| ln Vitro |
In healthy individuals, the median plasma concentration of taurodeoxycholate is 33.9 nM[1]. With an IC50 of 170 µM, taurodeoxycholate inhibits N-3H-methylscopolamine's ability to bind to the M3 muscarinic receptor of acetylcholine[1]. Taurodeoxycholate (0.05–1.00 mM; 1–6 days) promotes the growth of intestinal epithelial cells[2]. Taurodeoxycholate (0.05–1.00 mM; 24 h) enhances the production of c-myc protein and mRNA in IEC-6 cells and causes a significant drop in G1-phase concentration and increase in S-phase concentration of the cell cycle[2].
|
| ln Vivo |
Taurodeoxycholate (0.5 mg/kg; intravenous; once) protects sepsis-stricken C57BL/6N mice, but not TGR5 KO mice[1].
|
| Cell Assay |
Cell Proliferation Assay[2]
Cell Types: IEC-6 and caco-2 cells Tested Concentrations: 0, 0.05, 0.50, and 1.00 mM Incubation Duration: 1, 2, 4 and 6 days Experimental Results: Dramatically stimulated intestinal epithelial cell proliferation in a dose-dependent manner. Cell Cycle Analysis[2] Cell Types: IEC-6 cells Tested Concentrations: 0, 0.05, 0.50, and 1.00 mM Incubation Duration: 24 h Experimental Results: Dramatically increased cells in S phase and diminished cells in G1-phase. Western Blot Analysis[2] Cell Types: IEC-6 cells Tested Concentrations: 0.5 mM Incubation Duration: 1 and 6 days Experimental Results: Dramatically increased c-myc protein expression. |
| Animal Protocol |
Animal/Disease Models: C57BL/6N mice, Lipopolysaccharides injection model of sepsis[1]
Doses: 0.5 mg/kg Route of Administration: intravenous (iv) injection, 30 min or 24 h after LPS injection Experimental Results: Improved the survival rate of mice with sepsis. diminished liver and kidney damage in septic mice. Ameliorated systemic inflammation and normalized blood pressure in septic mice. |
| References | |
| Additional Infomation |
Sodium taurodeoxycholate hydrate is a cholanoid. It is functionally related to a taurodeoxycholic acid.
|
| Molecular Formula |
C26H44NNAO6S
|
|---|---|
| Molecular Weight |
521.69
|
| Exact Mass |
539.289
|
| CAS # |
207737-97-1
|
| Related CAS # |
Taurodeoxycholate sodium salt;1180-95-6;Taurodeoxycholic acid sodium hydrate;110026-03-4;Taurodeoxycholate-d6 sodium salt;2687960-92-3
|
| PubChem CID |
23702150
|
| Appearance |
White to off-white solid powder
|
| Melting Point |
168ºC (dec.)(lit.)
|
| LogP |
4.462
|
| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
7
|
| Rotatable Bond Count |
7
|
| Heavy Atom Count |
36
|
| Complexity |
864
|
| Defined Atom Stereocenter Count |
10
|
| SMILES |
C[C@H](CCC(=O)NCCS(=O)(=O)[O-])[C@H]1CC[C@@H]2[C@@]1([C@H](C[C@H]3[C@H]2CC[C@H]4[C@@]3(CC[C@H](C4)O)C)O)C.O.[Na+]
|
| InChi Key |
OLPIZAYYAVQETM-GGPRKOIFSA-M
|
| InChi Code |
InChI=1S/C26H45NO6S.Na.H2O/c1-16(4-9-24(30)27-12-13-34(31,32)33)20-7-8-21-19-6-5-17-14-18(28)10-11-25(17,2)22(19)15-23(29)26(20,21)3;;/h16-23,28-29H,4-15H2,1-3H3,(H,27,30)(H,31,32,33);;1H2/q;+1;/p-1/t16-,17-,18-,19+,20-,21+,22+,23+,25+,26-;;/m1../s1
|
| Chemical Name |
sodium;2-[[(4R)-4-[(3R,5R,8R,9S,10S,12S,13R,14S,17R)-3,12-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoyl]amino]ethanesulfonate;hydrate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O: 100 mg/mL (191.68 mM)
DMSO: 100 mg/mL (191.68 mM) |
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9168 mL | 9.5842 mL | 19.1685 mL | |
| 5 mM | 0.3834 mL | 1.9168 mL | 3.8337 mL | |
| 10 mM | 0.1917 mL | 0.9584 mL | 1.9168 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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