Size | Price | |
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100mg | ||
250mg | ||
500mg | ||
Other Sizes |
Targets |
Human Endogenous Metabolite
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ln Vitro |
Sodium (400 μM; 48 hours) prevents lipid peroxidation caused by indomethacin in Caco-2 cells [2]. When cells were treated with both indomethacin and uric acid (200 μM IND plus 400 μM UA; 24 hours), the amount of reactive oxygen species (ROS) was much lower than when cells were incubated with indomethacin alone. When Caco-2 cells were treated with both indomethacin and Uric acid sodium (200 μM IND plus 400 μM UA) for 24 hours, their cell viability was higher than when cells were only treated with indomethacin. Because of its antioxidant properties, sodium urate protects intestinal cells against alterations brought on by indomethacin [2].
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ln Vivo |
The enteropathy caused by indomethacin is improved by uric acid sodium (250 mg/kg; oral)[2]. In a mouse model of enteropathy produced by indomethacin, oral treatment of uric acid sodium decreases the formation of ROS in the ileum[2].
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Animal Protocol |
Animal/Disease Models: Eightweeks old male C57BL/6J mice[2]
Doses: 250 mg/kg body weight Route of Administration: Po Experimental Results: When mice treated with indomethacin were concurrently administered uric acid orally, ulcer areas were Dramatically decreased, in a uric acid dose-dependent manner. |
References |
[1]. Yasutake Y, et al. Uric acid ameliorates indomethacin-induced enteropathy in mice through its antioxidant activity. J Gastroenterol Hepatol. 2017;32(11):1839-1845.
[2]. Wang Q, et al. Recent Progress on Uric Acid Detection: A Review. Crit Rev Anal Chem. 2020;50(4):359-375. |
Molecular Formula |
C5H3N4NAO3
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Molecular Weight |
190.09
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CAS # |
1198-77-2
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Related CAS # |
Uric acid;69-93-2
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
[Na+].O=C1C2=C(N([H])C(N1[H])=O)[N-]C(N2[H])=O
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
1M NaOH: 8.33 mg/mL (43.82 mM)
DMSO: 3.12 mg/mL (16.41 mM) |
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 5.2607 mL | 26.3033 mL | 52.6067 mL | |
5 mM | 1.0521 mL | 5.2607 mL | 10.5213 mL | |
10 mM | 0.5261 mL | 2.6303 mL | 5.2607 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.