| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
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| ln Vitro |
Iron(III) citrate, or ferric citrate; 1 mM; 24 hr) dramatically causes CM cell death[1]. (CM) cells in a way that is dependent on dosage[1].
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|---|---|
| ln Vivo |
Here, we used age-matched wild-type mice and the Col4a3 deletion mouse model of progressive chronic kidney disease to examine the effects of ferric citrate (25 μg/g) supplementation against a mineral-sufficient control diet. For four weeks starting at six weeks of age when the knockout mice had overt chronic renal disease (CKD), or for six weeks starting at four weeks of age when the mice had early CKD, ferric citrate is administered to the mice. Regardless of when treatment is started, ferric citrate reverses iron shortage and anemia in knockout mice, and it also lowers the levels of FGF23 in the blood and on the bone. Additionally, ferric citrate enhances heart health and increases survival rates noticeably[3]. Ferric citrate is a safe and effective phosphate binder that preserves hemoglobin while increasing iron storage, decreasing the need for intravenous iron, reducing erythropoietin-stimulating drug use. Serum ferritin, hemoglobin, and transferrin saturation can all be raised by ferric citrate[2].
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| Cell Assay |
Cell Viability Assay[1]
Cell Types: Cardiomyocyte (CM) cells Tested Concentrations: 1 mM Incubation Duration: 24 hrs (hours) Experimental Results: Dramatically induced CM cell death. |
| References |
[1]. Yuichi Baba, et al. Protective effects of the mechanistic target of rapamycin against excess iron and ferroptosis in cardiomyocytes. Am J Physiol Heart Circ Physiol. 2018 Mar 1;314(3):H659-H668.
[2]. Julia B Lewis, et al. Ferric citrate controls phosphorus and delivers iron in patients on dialysis. J Am Soc Nephrol. 2015 Feb;26(2):493-503. [3]. Connor Francis, et al. Ferric citrate reduces fibroblast growth factor 23 levels and improves renal and cardiac function in a mouse model of chronic kidney disease. Kidney Int. 2019 Dec;96(6):1346-1358. |
| Molecular Formula |
C6H5FEO7
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|---|---|
| Molecular Weight |
244.94
|
| CAS # |
3522-50-7
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| Related CAS # |
Citric acid;77-92-9;Citric acid monohydrate;5949-29-1
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| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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| SMILES |
[Fe+3].O([H])C(C(=O)[O-])(C([H])([H])C(=O)[O-])C([H])([H])C(=O)[O-]
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 5 mg/mL (20.41 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 10 mg/mL (40.83 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.0826 mL | 20.4132 mL | 40.8263 mL | |
| 5 mM | 0.8165 mL | 4.0826 mL | 8.1653 mL | |
| 10 mM | 0.4083 mL | 2.0413 mL | 4.0826 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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