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100mg | ||
250mg | ||
500mg | ||
Other Sizes |
Targets |
IC50: 0.1 μM (Aldose reductase)[3]
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ln Vitro |
ICAM-1 expression at the mRNA and protein levels was not affected by Danshenol A (10 nM; pretreatment for 1 h) treatment alone. Danshenol A significantly reverses the elevated generation of reactive oxygen species (ROS) and NOX4 expression, as well as TNF-α-induced ICAM-1 expression and subsequent monocyte adhesion [1]. Danshenol A inhibits TNF-α-induced ICAM-1 expression and subsequent monocyte adhesion to endothelial cells through the NOX4-dependent IKKβ/NF-κB pathway [1]. Danshenol A (1, 3, and 10 μM; pretreated for 35 min) reverses the cardiomyocyte apoptosis caused by angiotensin II. Danshenol A also prevents cardiomyocytes from using the mitochondrial redox signaling pathway [2]. K562 (IC50 = 0.53 μg/mL), T-24 (IC50 = 7.94 μg/mL), QGY (IC50 = 4.65 μg/mL), and Me180 (IC50 = 6.89 μg/mL) cell lines are all inhibited by danshenol A [4].
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ln Vivo |
In SHR rats, Danshenol A (0.3–3 mg/kg; oral; once daily; for 12 weeks) increases cardiac function, myocardial collagen volume, and blood pressure. Danshenol A lowers cardiac oxidative stress and restores the structure and function of mitochondria[2].
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Cell Assay |
Western Blot Analysis[1]
Cell Types: HUVEC cells Tested Concentrations: 10 nM Incubation Duration:Pretreatment for 1 h Experimental Results: demonstrated no effect on the ICAM-1 expression at both mRNA and protein levels. |
Animal Protocol |
Animal/Disease Models: Forty male spontaneously hypertensive rats (SHR) and eight male Wistar-Kyoto (WKY) rats at the age of 16 weeks[2]
Doses: 0.3 mg/kg, 1 mg/kg, 3 mg/kg Route of Administration: Orally administration ; daily; for 12 weeks Experimental Results: Ameliorated blood pressure, cardiac injury, and myocardial collagen volume and improved cardiac function. |
References |
[1]. Wenwen Zhao, et al. Danshenol A inhibits TNF-α-induced expression of intercellular adhesion molecule-1 (ICAM-1) mediated by NOX4 in endothelial cells. Sci Rep. 2017 Oct 11;7(1):12953.
[2]. Kai Chen, et al. Danshenol A Alleviates Hypertension-Induced Cardiac Remodeling by Ameliorating Mitochondrial Dysfunction and Suppressing Reactive Oxygen Species Production. Oxid Med Cell Longev. 2019 Sep 11;2019:2580409. [3]. Y Tezuka, et al. Aldose reductase inhibitory constituents of the root of Salvia miltiorhiza Bunge. Chem Pharm Bull (Tokyo). 1997 Aug;45(8):1306-11. [4]. Gang Xu, et al. Two new abietane diterpenoids from Salvia yunnanensis. Planta Med. 2006 Jan;72(1):84-6. |
Molecular Formula |
C21H20O4
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Molecular Weight |
336.38
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CAS # |
189308-08-5
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
CC1=C2C=CC3=C(C2=CC=C1)[C@@](CC(=O)C)(C(=O)C4=C3OC[C@@H]4C)O
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.9728 mL | 14.8641 mL | 29.7283 mL | |
5 mM | 0.5946 mL | 2.9728 mL | 5.9457 mL | |
10 mM | 0.2973 mL | 1.4864 mL | 2.9728 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.