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250mg | ||
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Other Sizes |
ln Vitro |
Compound 14q, also known as antioxidant agent-3, demonstrates good activity in scavenging DPPH radicals and ABTS +•, with IC50 values of 26.58 μM and 30.31 μM, respectively[1]. When tested on human normal WI-38 (IC50 > 100 μM) and GES (IC50 > 200 μM) cells, antioxidant agent-3 exhibits minimal cytotoxicity[1]. HepG2 cells induced by H2O2 can have their viability increased by antioxidant agent-3[1]. HepG2 cells' percentage of apoptotic cells is decreased by antioxidant agent-3[1]. In H2O2-treated HepG2 cells, antioxidant agent-3 decreases ROS production and LDH release, raises GSH and SOD levels, and increases stability in methanol solution[1].
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Cell Assay |
Cell Cytotoxicity Assay[1]
Cell Types: WI-38 and GES cells line Tested Concentrations: 200, 100, 50, 25 and 12.5 μM Incubation Duration: 48 h Experimental Results: demonstrated less toxicity in hemolysis assay and weaker antiproliferative effects. Cell Viability Assay[1] Cell Types: H2O2-damaged WI-38 and HepG2 cells Tested Concentrations: 50, 25 and 12.5 μM Incubation Duration: 1 h Experimental Results: Increased cells viability of H2O2-indcued cells and protected the H2O2-indcued cells against injury. Apoptosis Analysis[1] Cell Types: H2O2-damaged HepG2 cells Tested Concentrations: 50, 25 and 12.5 μM Incubation Duration: 1 h Experimental Results: Protect the H2O2-injured HepG2 cells against apoptosis through antioxidant effect. |
References |
[1]. Wen-Bo Li, et al.Synthesis and antioxidant activity of conjugates of hydroxytyrosol and coumarin. Bioorg Chem. 2020 Dec;105:104427.
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Molecular Formula |
C18H14O8
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Molecular Weight |
358.30
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CAS # |
2710376-48-8
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.7910 mL | 13.9548 mL | 27.9096 mL | |
5 mM | 0.5582 mL | 2.7910 mL | 5.5819 mL | |
10 mM | 0.2791 mL | 1.3955 mL | 2.7910 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.