Size | Price | Stock | Qty |
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5mg |
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10mg |
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Other Sizes |
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ln Vitro |
YL-939 (0.01-10 μM) and HT-1080, Miapaca-2, Calu-1, HCT116, and SHSY5Y cells significantly protect cells against ferroptosis (IC50 values of 0.14 μM, 0.25 μM, 0.16 μM, 0.16 μM). 0.24 micrograms per milligram[1]. Reduces ROS levels in cytosol and membrane lipids with YL-939 (5 μM; 10 hours; ES-2 cells)[1]. PHB2 is a biological target of YL-939 (5 μM; 1-10 h; ES-2 cells) [1]. The concentration-dependent inhibition of ferritin autophagy is achieved by YL-939 (3 μM; 10 h; ES-2 cells) through its improvement of ferritin expression and its ability to block autophagosomes/lysosomes.
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ln Vivo |
In an acute liver injury model induced by acetaminophen (APAP), YL-939 (3 mg/kg; i.p.; single injection) ameliorates liver injury [1].
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Cell Assay |
Western Blot Analysis[1]
Cell Types: ES-2 cells Tested Concentrations: 5 μM Incubation Duration: 1, 5, 7.5, and 10 hrs (hours) Experimental Results: Pulled down PHB2 protein by the probe. Western Blot Analysis[1] Cell Types: ES- 2 cells Tested Concentrations: 3 μM Incubation Duration: 10 hrs (hours) Experimental Results: Increased the expression of nuclear receptor coactivator 4(NCOA4) in a dose-dependent manner. |
Animal Protocol |
Animal/Disease Models: Acetaminophen (APAP)-induced male C57BL /6J mouse[1]
Doses: 3 mg/kg Route of Administration: intraperitoneal (ip)injection; single injection Experimental Results: Inhibited the cell death and inflammatory infiltration in the liver tissues of male C57BL/J6 mice that received APAP. |
References |
[1]. Yang W, et, al. Non-classical ferroptosis inhibition by a small molecule targeting PHB2. Nat Commun. 2022 Dec 3;13(1):7473.
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Molecular Formula |
C25H26N6O
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Molecular Weight |
426.51
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO :~100 mg/mL (~234.46 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.86 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.3446 mL | 11.7231 mL | 23.4461 mL | |
5 mM | 0.4689 mL | 2.3446 mL | 4.6892 mL | |
10 mM | 0.2345 mL | 1.1723 mL | 2.3446 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.