| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
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| Targets |
TLR4 1.675 μM (IC50, in HEK293 cells)
|
|---|---|
| ln Vitro |
In HEK293 cells, lipid A-induced increases in phosphatase activity are inhibited in a concentration-dependent manner by CAY10614 (Compound 7) (1–10 μM) [1]. In neurons cultured for more than 18 days in vitro (DIV), CAY10614 (0.5 μM) suppresses the rise in [Ca2+]cyt brought on by LPS [2].
|
| ln Vivo |
Mice given intraperitoneal LPS (20 mg/kg) had considerably higher survival rates when CAY10614 (Compound 7) (10 mg/kg; intraperitoneally injected 30 minutes before LPS) is administered[1].
|
| Animal Protocol |
Animal/Disease Models: C57BL/6J male mice (9 weeks) were ip injected with 20 mg/kg LPS[1]
Doses: 10 mg/kg Route of Administration: Ip 30 min before the LPS Experimental Results: Increased the survival rate of mice from 0% to 67%. |
| References |
|
| Molecular Formula |
C42H78INO2
|
|---|---|
| Molecular Weight |
755.98
|
| Exact Mass |
755.507
|
| CAS # |
1202208-36-3
|
| PubChem CID |
25223004
|
| Appearance |
White to off-white solid powder
|
| LogP |
10.369
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
31
|
| Heavy Atom Count |
46
|
| Complexity |
630
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
UXOPVNPWTUWUEA-UHFFFAOYSA-M
|
| InChi Code |
InChI=1S/C42H78NO2.HI/c1-5-7-9-11-13-15-17-19-21-23-25-29-35-44-41-34-33-39(38-43(3,4)40-31-27-28-32-40)37-42(41)45-36-30-26-24-22-20-18-16-14-12-10-8-6-2;/h33-34,37,40H,5-32,35-36,38H2,1-4H3;1H/q+1;/p-1
|
| Chemical Name |
cyclopentyl-[[3,4-di(tetradecoxy)phenyl]methyl]-dimethylazanium;iodide
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 12.5 mg/mL (16.53 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 0.83 mg/mL (1.10 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.3 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 0.83 mg/mL (1.10 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.3 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3228 mL | 6.6139 mL | 13.2279 mL | |
| 5 mM | 0.2646 mL | 1.3228 mL | 2.6456 mL | |
| 10 mM | 0.1323 mL | 0.6614 mL | 1.3228 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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