| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
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| ln Vitro |
Hippocampal pyramidal neurons' excitability is inhibited by Ro 5212773 (EPPTB). Additionally, EPPTB lowers seizure activity and epileptiform events (SLE) [1]. Inward rectifier K+ current activation mediated by TAAR1 is blocked by Ro 5212773 [2]. 1.5 μM β-phenylethylamine (IC50=27.5 nM) potently inhibits the production of cAMP induced by mouse TAAR1 activation. This is achieved by Ro 5212773. In the absence of TAAR1 agonist, Ro 5212773 dose-dependently lowers cAMP levels in HEK293 cells (IC50= 19 nM). Compared to rat (IC50= 4539 nM) and human (IC50= 7487 nM) TAAR1, EPPTB significantly more effectively inhibits the production of cAMP in mice [2].
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| References |
| Molecular Formula |
C20H21F3N2O2
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|---|---|
| Molecular Weight |
378.39
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| Exact Mass |
378.156
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| CAS # |
1110781-88-8
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| PubChem CID |
25175634
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| Appearance |
White to off-white solid powder
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| LogP |
5.094
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
27
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| Complexity |
494
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| Defined Atom Stereocenter Count |
0
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (264.28 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.61 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6428 mL | 13.2139 mL | 26.4278 mL | |
| 5 mM | 0.5286 mL | 2.6428 mL | 5.2856 mL | |
| 10 mM | 0.2643 mL | 1.3214 mL | 2.6428 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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