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25g |
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Other Sizes |
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ln Vitro |
At lower concentrations of 40 μg/mL, κ-Car-Curcumin (Cur) (0-500 μg/mL; 24-72 hours) significantly inhibits the development of cancer cells[1]. In certain A549 lung cancer cells, the cytotoxicity of the Cur-loaded κ-Car dramatically increases apoptotic activity[1]. In HT-29 cells, κ-carrageenan (1–60 μg/mL; 0.5–24 hours) increases LPS-induced IL-8 secretion[2].
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ln Vivo |
κ-Carrageenan can be applied to animal modeling to create models of edema in the feet of rats and mice.
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Cell Assay |
Cell Viability Assay[1]
Cell Types: A549 cells Tested Concentrations: 0-500 μg/mL Incubation Duration: 24, 48 and 72 hrs (hours) Experimental Results: The dose response effects of cells treated with Cur loaded κ-Car after incubation of 24, 48 and 72 h demonstrated significant IC50 values of 65, 50 and 40 μg/mL respectively, for 24, 48, 72 h ours. |
Animal Protocol |
Animal/Disease Models: Male and female NIH (s) mice[2]
Doses: 1.7 mg/kg, LOW; 8.3 mg/kg, MED; or 41.7 mg/kg, HIG Route of Administration: Orally administered for 1 week prior to C. freundii DBS100 treatment Experimental Results: Enhanced the C. freundii DBS100-dependent induction of TLR4 and NF-κB in the intestinal mucosa of infected mice. |
References |
[1]. Sathuvan M, et al. κ-Carrageenan: An effective drug carrier to deliver curcumin in cancer cells and to induce apoptosis. Carbohydr Polym. 2017;160:184-193.
[2]. Wu W, et al. κ-Carrageenan Enhances Lipopolysaccharide-Induced Interleukin-8 Secretion by Stimulating the Bcl10-NF-κB Pathway in HT-29 Cells and Aggravates C. freundii-Induced Inflammation in Mice. Mediators Inflamm. 2017;2017:8634865. [3]. Wei W, et al. Enhanced effect of κ-carrageenan on TNBS-induced inflammation in mice. Int Immunopharmacol. 2016;39:218-228. |
CAS # |
11114-20-8
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
H2O: 8.33 mg/mL
DMSO: 8.33 mg/mL |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 0.83 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.3 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 0.83 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.3 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 0.83 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 8.33 mg/mL (Infinity mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication (<60°C). |
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.