Size | Price | Stock | Qty |
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5mg |
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10mg |
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Other Sizes |
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ln Vitro |
At an IC50 value of 100 μM, HOIPIN-8 (0-100 μM; 72 hours) exhibits nearly little cytotoxicity against A549 cells[1]. HOIPIN-8 (0–10 μM; 24 hours) inhibits NF-κB activation more than ten times more than HOIPIN-1, which has an IC50 value of 0.42 μM in HEK293T cells [1]. With an IC50 value of 11.9 μM, HOIPIN-8 (0-30 μM) is 4 times more successful than HOIPIN-1, which transfects cells with the NF-κB luciferase reporter gene for 18 hours before transfecting them with 10 ng/mL TNF-α for 6 hours. It is also more effective than HOIPIN-1 at reducing the expression of NF-κB target genes (including ICAM1 and IL-6) that are triggered by IL-1β [1].
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Cell Assay |
Cell Viability Assay[1]
Cell Types: Human lung carcinoma A549 cells, HEK293T cells Tested Concentrations: 0 μM; 1 μM; 3 μM; 10 μM; 30 μM; 100 μM Incubation Duration: 72 hrs (hours) Experimental Results: Did not effect A549 cell viability. RT-PCR[1] Cell Types: HEK293T cells Tested Concentrations: 0 μM; 0.3 μM; 1 μM; 10 μM; 30 μM Incubation Duration: 24 hrs (hours) Experimental Results: Had an inhibitory effect on the inflammatory cytokine-induced NF-κB activation pathway. |
References |
[1]. Ken Katsuya, et al. Small-molecule Inhibitors of Linear Ubiquitin Chain Assembly Complex (LUBAC), HOIPINs, Suppress NF-κB Signaling. Biochem Biophys Res Commun. 2019 Feb 12;509(3):700-706.
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Molecular Formula |
C23H15F2N4NAO3
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Molecular Weight |
456.38
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO :~100 mg/mL (~219.12 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: 1.25 mg/mL (2.74 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (2.74 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: 1.25 mg/mL (2.74 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.1912 mL | 10.9558 mL | 21.9116 mL | |
5 mM | 0.4382 mL | 2.1912 mL | 4.3823 mL | |
10 mM | 0.2191 mL | 1.0956 mL | 2.1912 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.