| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
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| ln Vitro |
The quantities of active GLP-1(7-36) Acetate (Human GLP-1-(7-36)-amide Acetate) in the medium were considerably greater in the cells treated with phorbol 12-myristate 13-acetate for two hours compared to the control group. Additionally, in a dose-dependent manner, the glucose administration raises the active GLP-1 release from cells. Active GLP-1 secretion from cells was dose-dependently enhanced by palmitic, oleic, linoleic, or linolenic acid. Compared to palmitic acid, unsaturated fatty acids including oleic, linoleic, and linolenic acids considerably increase active GLP-1 production. Active GLP-1 concentrations in the medium are dose-dependently increased when NCI-H716 cells are treated with CPE. At a concentration of 0.1% CPE, there is a 37% increase in these cells' active GLP-1 secretion[1].
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| ln Vivo |
Active GLP-1(7-36) amide levels in the portal blood rise following gastric glucose delivery after 10 minutes, and then they sharply decline after 30 minutes. After 10 minutes, the stomach delivery of TO also raises active GLP-1 levels, which subsequently drop to basal levels after 60 minutes. The secretion of GLP-1 is dose-dependently increased by glucose and TO alone. Moreover, peak GLP-1 levels are additively increased by the simultaneous administration of glucose and TO. Within the portal blood at 10 and 30 minutes, mice given CPE had elevated amounts of active GLP-1 compared to the control group. When CPE is given in addition to glucose, the mice receiving CPE have somewhat higher levels of insulin and active GLP-1 in their portal blood than the mice receiving no CPE. C57BL/6J mice fed a high-fat diet experience glucose intolerance impairment and hyperglycemia [1].
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| References |
[1]. Fujii Y et al. Ingestion of coffee polyphenols increases postprandial release of the active glucagon-like peptide-1(GLP-1(7-36)) amide in C57BL/6J mice. J Nutr Sci. 2015 Mar 3;4:e9.
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| Molecular Formula |
C151H227F3N40O47
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|---|---|
| Molecular Weight |
3411.65
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| Related CAS # |
GLP-1(7-36), amide acetate;1119517-19-9;GLP-1(7-36), amide;107444-51-9
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| Appearance |
Typically exists as solid at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO :≥ 50 mg/mL (~14.66 mM)
H2O :< 0.1 mg/mL |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (0.37 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (0.37 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.25 mg/mL (0.37 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.2931 mL | 1.4656 mL | 2.9311 mL | |
| 5 mM | 0.0586 mL | 0.2931 mL | 0.5862 mL | |
| 10 mM | 0.0293 mL | 0.1466 mL | 0.2931 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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