Size | Price | Stock | Qty |
---|---|---|---|
1mg |
|
||
5mg |
|
||
10mg |
|
||
Other Sizes |
|
ln Vitro |
According to the study, Cypate coupled upconversion nanoparticles (UCNP-cy) loaded with anti-heat shock protein 70 small interfering RNA genes (UCNP-cy-siRNA) can cause targeted cellular damage and have anti-tumor properties [1]. In order to track, label, and photograph particular biomolecules or cells, Cypate can also be connected to CBT (Cysteine-containing Peptide Backbone Tag). For instance, cathepsin B (CTSB) activity can be specifically seen and tracked in real time in tumors and cells that overexpress CTSB using Cypate-CBT, a near-infrared photoacoustic (PA) probe. Cypate-CBT increases the strength and duration of the PA signal at the tumor site by entering CTSB-overexpressing cells, where it is then subjected to glutathione reduction and CTSB cleavage to produce cypate nanoparticles, or Cypate-CBT-NPs. For the clinical diagnosis of early cancer, cipate-CBT has the potential to be a useful PA imaging agent [2].
|
---|---|
References |
[1]. Wang L, et al. Cypate-conjugated porous upconversion nanocomposites for programmed delivery of heat shock protein 70 small interfering RNA for gene silencing and photothermal ablation[J]. Advanced Functional Materials, 2016, 26(20): 3480-3489.
[2]. Wang C, et al. Cathespin B-Initiated Cypate Nanoparticle Formation for Tumor Photoacoustic Imaging. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202114766. |
Molecular Formula |
C41H41CLN2O4
|
---|---|
Molecular Weight |
661.23
|
Related CAS # |
Cypate;95837-47-1
|
Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
|
HS Tariff Code |
2934.99.9001
|
Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
Solubility (In Vitro) |
DMSO :~12.5 mg/mL (~18.90 mM)
H2O :< 0.1 mg/mL |
---|---|
Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (1.89 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 1.25 mg/mL (1.89 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.5123 mL | 7.5617 mL | 15.1233 mL | |
5 mM | 0.3025 mL | 1.5123 mL | 3.0247 mL | |
10 mM | 0.1512 mL | 0.7562 mL | 1.5123 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.