Size | Price | Stock | Qty |
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5mg |
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10mg |
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Other Sizes |
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Targets |
NMDA Receptor
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ln Vitro |
The saffron metabolite crocetin (Transcrocetin) meglumine, which comes from the crocin apocarotenoids, has been demonstrated to have a significant affinity for NMDA receptors and is assumed to be the cause of saffron's central nervous system activity.After a 24-hour incubation period with the test compounds, the cellular mitochondrial dehydrogenase activity of Caco-2 cells is measured by the MTT assay to ensure that the viability of the cells remains unchanged throughout the transport experiments. The results show that neither hydroalcoholic saffron extract (SE, 0.5-1 mg/mL) nor crocin-1 (250-1000 µM) significantly alter the viability of the cells. Cell viability is not affected by crocetin meglumine at 10 µM doses, but it is dramatically reduced at higher concentrations (40–160 µM)[1].
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References |
[1]. Lautenschläger M, et al. Intestinal formation of trans-Crocetin from saffron extract (Crocus sativus L.) and in vitro permeation through intestinal and blood brain barrier. Phytomedicine. 2015 Jan 15;22(1):36-44.
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Molecular Formula |
C34H58N2O14
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Molecular Weight |
718.83
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Related CAS # |
Crocetin disodium;591230-99-8;Crocetin;27876-94-4
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO :~8.33 mg/mL (~11.59 mM )
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Solubility (In Vivo) |
Solubility in Formulation 1: 0.83 mg/mL (1.15 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.3 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 0.83 mg/mL (1.15 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.3 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.3911 mL | 6.9557 mL | 13.9115 mL | |
5 mM | 0.2782 mL | 1.3911 mL | 2.7823 mL | |
10 mM | 0.1391 mL | 0.6956 mL | 1.3911 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.