| Size | Price | |
|---|---|---|
| Other Sizes |
| ln Vitro |
Glycodeoxycholic Acid (200 μM, 24-48 h) induces stemness and chemoresistance of hepatocellular carcinoma cells via the STAT3 signaling pathway[1]. Glycodeoxycholic Acid (50 μM, pretreatment for 1 h) abolishes UCB-induced cytochrome c oxidase inhibition and significantly prevents oxidative stress, metabolic changes, and cell death[2].
|
|---|---|
| ln Vivo |
Glycodeoxycholic Acid (11.20 mg/kg, injected into the bile-pancreatic duct) induces acute pancreatitis in macaques[3].
|
| Cell Assay |
Cell Viability Assay[1]
Cell Types: Huh7, LM3 Concentration: 200 μM Incubation Duration: 24, 48 h Experimental Results: Increased cell viability treated with 5-FU and cisplatin. Western Blot Analysis[1] Cell Types: Huh7, LM3 Concentration: 200 μM Incubation Duration: 24, 48 h Experimental Results: Suppressed the expression of apoptotic genes and increased anti-apoptotic genes. Promoted the expression of Sox2, Sox9, Nanog and CD133. Down-regulated the level of E-cadherin and up-regulated vimentin. Decreased the levels of SOCS2, SOCS5, PTPN1 and PTPN11. |
| Animal Protocol |
Animal/Disease Models:Experimental macaque model[3]
Doses: 11.20 mg/kg Route of Administration: injected along the biliopancreatic duct Experimental Results: Increased the levels of Serum amylase and lipase. Elevated Blood pressure and heart rate. |
| References |
|
| Additional Infomation |
Glycodeoxycholic acid is a bile acid glycine conjugate of deoxycholic acid. It has a role as a human metabolite. It is functionally related to a deoxycholic acid. It is a conjugate acid of a glycodeoxycholate.
Glycodeoxycholic acid has been reported in Streptomyces nigra, Trypanosoma brucei, and Caenorhabditis elegans with data available. A bile salt formed in the liver by conjugation of deoxycholate with glycine, usually as the sodium salt. It acts as a detergent to solubilize fats for absorption and is itself absorbed. It is used as a cholagogue and choleretic. |
| Molecular Formula |
C26H43NO5
|
|---|---|
| Molecular Weight |
449.62
|
| Exact Mass |
467.325
|
| CAS # |
360-65-6
|
| PubChem CID |
3035026
|
| Appearance |
Solid powder
|
| Density |
1.162 g/cm3
|
| Boiling Point |
655.6ºC at 760 mmHg
|
| Flash Point |
350.3ºC
|
| Index of Refraction |
1.546
|
| LogP |
3.92
|
| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
6
|
| Heavy Atom Count |
32
|
| Complexity |
727
|
| Defined Atom Stereocenter Count |
10
|
| SMILES |
O([H])[C@@]1([H])C([H])([H])[C@@]2([H])[C@@]3(C([H])([H])[H])C([H])([H])C([H])([H])[C@]([H])(C([H])([H])C3([H])C([H])([H])C([H])([H])[C@@]2([H])[C@]2([H])C([H])([H])C([H])([H])[C@]([H])([C@]([H])(C([H])([H])[H])C([H])([H])C([H])([H])C(N([H])C([H])([H])C(=O)O[H])=O)[C@]21C([H])([H])[H])O[H]
|
| InChi Key |
WVULKSPCQVQLCU-BUXLTGKBSA-N
|
| InChi Code |
InChI=1S/C26H43NO5/c1-15(4-9-23(30)27-14-24(31)32)19-7-8-20-18-6-5-16-12-17(28)10-11-25(16,2)21(18)13-22(29)26(19,20)3/h15-22,28-29H,4-14H2,1-3H3,(H,27,30)(H,31,32)/t15-,16-,17-,18+,19-,20+,21+,22+,25+,26-/m1/s1
|
| Chemical Name |
2-[[(4R)-4-[(3R,5R,8R,9S,10S,12S,13R,14S,17R)-3,12-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoyl]amino]acetic acid
|
| Synonyms |
Glycodeoxycholic acid; Glycodeoxycholic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : 125 mg/mL (278.01 mM; with sonication (<60°C))
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (4.63 mM)(Saturation unknown) in 10% DMSO 40% PEG300 5% Tween-80 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution, add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix well; then add 50 μL Tween-80 to the above system and mix well; then add 450 μL saline to make up to 1 mL. *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (4.63 mM)(Saturation unknown) in 10% DMSO 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution, add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD in saline and mix well. *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.08 mg/mL (4.63 mM)(Saturation unknown) in 10% DMSO 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution, add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2241 mL | 11.1205 mL | 22.2410 mL | |
| 5 mM | 0.4448 mL | 2.2241 mL | 4.4482 mL | |
| 10 mM | 0.2224 mL | 1.1121 mL | 2.2241 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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