RNA synthesis, which is also called DNA transcription, is a highly selective process.Beyond RNA synthesis, RNA polymerase II transcription plays a more active role in the maturation, monitoring, and export of mRNA to the cytoplasm.
In order to fully repair the DNA after a single-strand break, DNA ligase constructs the final phosphodiester bond using the complementary strand of the double helix as a template.Substrates with RNA strands or mismatched base pairs near the ends of the nicked DNA are discriminated against by DNA ligases. Bleomycin (BLM) is a S-independent radiomimetic agent that damages DNA by creating free radicals that attack the C-4′ position in the deoxyribose backbone. This causes the ribose ring to open and strand breaks to occur.
M-MuLV Reverse Transcriptase (RNase H) and random hexamer primers are used to create first strand cDNA. The next step involves the use of DNA Polymerase I and RNase H to create second strand cDNA. Exonuclease/polymerase activity transforms the remaining overhangs into blunt ends. DNA fragments' 3′ ends are adenylated before being ligated with a NEBNext Adaptor with a hairpin loop structure to get the samples ready for hybridization. The two pathways that BET bromodomain inhibition most commonly affects are cell cycle and DNA replication. The translation of mRNA into proteins is inhibited by cycloheximide.
| Structure | Cat No. | Product Name | CAS No. | Product Description |
|---|---|---|---|---|
|
V53093 | DMT-dA(bz) Phosphoramidite (DA-CE phosphoramidite) | 98796-53-3 | DMT-dA(bz) Phosphoramidite is widely used to synthesize DNA. |
|
V56557 | DMT-dA(bz) Phosphoramidite-13C10,15N5 (DA-CE phosphoramidite-13C10,15N5) | 2483830-15-3 | DMT-dA(bz) Phosphoramidite-13C10,15N5 is 13C and 15N labeled DMT-dA(bz) Phosphoramidite. |
|
V79179 | DMT-dA(bz) Phosphoramidite-15N5 (DA-CE phosphoramidite-15N5) | DMT-dA(bz) Phosphoramidite-15N5 is 15N (nitrogen 15) labelled DMT-dA(bz) Phosphoramidite. | |
|
V53092 | DMT-dA(PAc) Phosphoramidite | 110543-74-3 | DMT-dA(PAc) Phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of DNA or RNA. |
|
V79180 | DMT-dG(ib) Phosphoramidite-15N5 | DMT-dG(ib) Phosphoramidite-15N5 is 15N labeled DMT-dG(ib) Phosphoramidite. | |
|
V52929 | DMT-dT Phosphoramidite | 98796-51-1 | DMT-dT Phosphoramidite is widely used to synthesize DNA. |
|
V56553 | DMT-dT Phosphoramidite-13C10,15N2 | 2483830-17-5 | DMT-dT Phosphoramidite-13C10,15N2 is 13C and 15N labeled DMT-dT Phosphoramidite. |
|
V79181 | DMT-dT Phosphoramidite-15N2 | DMT-dT Phosphoramidite-15N2 is 15N labeled DMT-dT Phosphoramidite. | |
|
V52172 | DMT-dU-CE Phosphoramidite | 109389-30-2 | DMT-dU-CE Phosphoramidite is a nucleoside molecule that may be utilized in DNA synthesis and DNA sequencing. |
|
V55196 | DMT-LNA-5mA phosphoramidite | 1184723-53-2 | (1R,3R,4R,7S)-3-(4-Amino-5-methyl-2-oxopyrimidin-1(2H)-yl)-1-((bis(4-methoxyphenyl)(phenyl)methoxy)methyl) -2,5-dioxabicyclo[2.2.1]heptan-7-yl (2-cyanoethyl) diisopropylphosphoramidite is a phosphoramidite monomer that may be utilized in the solid-phase synthesis of oligonucleotides. |
|
V55179 | DMT-LNA-G phosphoramidite | 207131-17-7 | DMT-LNA-G phosphoramidite is a phosphoramidite monomer that can be used for solid-phase synthesis of oligonucleotides. |
|
V55165 | DMT-locG(ib) Phosphoramidite | 206055-77-8 | DMT-locG(ib) Phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides. |
|
V55185 | DMT-locMeC(bz) phosphoramidite | 206055-82-5 | DMT-locMeC(bz) phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides. |
|
V55172 | DMTr-FNA-C(Bz)Phosphoramidite | 326802-62-4 | DMTr-FNA-C(Bz)Phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides. |
|
V55173 | DMTr-LNA-U-3-CED-Phosphora | 206055-76-7 | DMTr-LNA-U-3-CED-phosphora is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides. |
|
V79183 | DMTr-MOE-Inosine-3-CED-phosphoramidite | DMTr-MOE-Inosine-3-CED-phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides. | |
|
V75903 | DMTr-TNA-5MeU-amidite | 325683-94-1 | DMTr-TNA-5MeU-amidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides. |
|
V55191 | DMTr-TNA-U-amidite | 325683-95-2 | DMTr-TNA-U-amidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides. |
|
V53564 | DNA gyrase B-IN-3 | 2412834-56-9 | DNA gyrase B-IN-3 (Compound A) is a bacterial DNA gyrase B inhibitor (IC50: <10 nM). |
|
V53681 | DNA Gyrase-IN-7 | 2925308-68-3 | DNA Gyrase-IN-7 (compound 6d) is a microbial DNA gyrase inhibitor. |
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