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DNA(RNA) Synthesis

DNA(RNA) Synthesis

RNA synthesis, which is also called DNA transcription, is a highly selective process.Beyond RNA synthesis, RNA polymerase II transcription plays a more active role in the maturation, monitoring, and export of mRNA to the cytoplasm.

In order to fully repair the DNA after a single-strand break, DNA ligase constructs the final phosphodiester bond using the complementary strand of the double helix as a template.Substrates with RNA strands or mismatched base pairs near the ends of the nicked DNA are discriminated against by DNA ligases. Bleomycin (BLM) is a S-independent radiomimetic agent that damages DNA by creating free radicals that attack the C-4′ position in the deoxyribose backbone. This causes the ribose ring to open and strand breaks to occur.

M-MuLV Reverse Transcriptase (RNase H) and random hexamer primers are used to create first strand cDNA. The next step involves the use of DNA Polymerase I and RNase H to create second strand cDNA. Exonuclease/polymerase activity transforms the remaining overhangs into blunt ends. DNA fragments' 3′ ends are adenylated before being ligated with a NEBNext Adaptor with a hairpin loop structure to get the samples ready for hybridization. The two pathways that BET bromodomain inhibition most commonly affects are cell cycle and DNA replication. The translation of mRNA into proteins is inhibited by cycloheximide.

DNA(RNA) Synthesis related products

Structure Cat No. Product Name CAS No. Product Description
m7(3'OMeG)(5')ppp(5')m6(2'OMeA)pG trisodium V90341 m7(3'OMeG)(5')ppp(5')m6(2'OMeA)pG trisodium 2956651-00-4 m7(3'OMeG)(5')ppp(5')m6(2'OMeA)pG trisodium is a cap analog that can be used for in vitro transcription of mRNA.
m7G(5')ppp(5')(2'OMeA)pU ammonium V90340 m7G(5')ppp(5')(2'OMeA)pU ammonium m7G(5')ppp(5')(2'OMeA)pU ammonium is a cap analog that can be used for in vitro transcription of mRNA.
m7GpppAmpG ammonium V76799 m7GpppAmpG ammonium m7GpppAmpG ammonium is a trinucleotide 5′ end cap analog that caps RNA with 90% efficiency.
m7GpppApG triammonium V94606 m7GpppApG triammonium m7GpppApG triammonium is a trinucleotide that is an analog of the mRNA 5' cap and can be used for in vitro RNA synthesis.
m7GpppGmpG ammonium V80658 m7GpppGmpG ammonium m7GpppGmpG ammonium is a trinucleotide 5′ cap analog.
m7Gpppm6AmpG ammonium V80659 m7Gpppm6AmpG ammonium m7Gppm6AmpG ammonium is a trinucleotide mRNA 5' cap analog that can be used for in vitro RNA synthesis.
Madrasin V24915 Madrasin 374913-63-0 Madrasin is a potent and cell penetrant splicing inhibitor that interferes with the early stages of spliceosome assembly.
Maleic hydrazide-d2 (抑芽丹-d2；马来酰肼-d2；青鲜素-d2) V56545 Maleic hydrazide-d2 (maleic hydrazide-d2; maleic hydrazide-d2) 2398483-97-9 Maleic hydrazide-d2 is the deuterated form of Maleic hydrazide.
Mequindox V52998 Mequindox 13297-17-1 Mequindox is a novel and seletive antibacterial agent. Mequindox inhibits the synthesis of DNA.
ML216 (CID-49852229) V4019 ML216 (CID-49852229) 1430213-30-1 ML216 (formerly known as CID-49852229) is a novel potent inhibitor of the Blooms syndrome protein (BLM) helicase which is responsible for DNA unwinding activity.
MMV688845 ((R)-GSK1729177A) V41179 MMV688845 ((R)-GSK1729177A) 2208962-35-8 MMV688845 is a non-tuberculous mycobacterial RNA polymerase inhibitor (antagonist) with bactericidal ( bacteria killing) activity against Mycobacterium abscessus and anti-tuberculosis (TB) activity.
Mn007 V103880 Mn007 1843236-92-9 Mn007 is a potent bovine pancreatic DNase I inhibitor, primarily through the formation of molecular aggregates, with IC50 of 45 µM.
Moloney murine leukemia virus RT V80805 Moloney murine leukemia virus RT Moloney murine leukemia virus RT is a monomeric reverse transcriptase derived from Moloney murine leukemia virus (MMLV).
Mulnitorsen V55138 Mulnitorsen 2576358-63-7 Mulnitorsen is an inhibitor (blocker/antagonist) of antisense non-coding mitochondrial RNA (ASncmtRNA) synthesis.
N,N-Dimethyl-ATP sodium V88657 N,N-Dimethyl-ATP sodium N,N-Dimethyl-ATP sodium is a labeled modified deoxyoligonucleotide (dNTP) that can release pyrophosphate to produce fluorescence and has special applications in gene synthesis and sequencing.
N-DMTr-Morpholino-T-5'-O-phosphoramidite V80917 N-DMTr-Morpholino-T-5'-O-phosphoramidite N-DMTr-Morpholino-T-5'-O-phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides.
N-DMTr-Morpholino-U-5'-O-phosphoramidite V80918 N-DMTr-Morpholino-U-5'-O-phosphoramidite N-DMTr-Morpholino-U-5'-O-phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides.
N-DMTr-N2-Isobutyryl-morpholino-G-5'-O-phosphoramidite V80919 N-DMTr-N2-Isobutyryl-morpholino-G-5'-O-phosphoramidite N-DMTr-N2-Isobutyryl-morpholino-G-5'-O-phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides.
N-DMTr-N4-Benzoyl-morpholino-cytosine-5'-O-phosphoramidite V80920 N-DMTr-N4-Benzoyl-morpholino-cytosine-5'-O-phosphoramidite N-DMTr-N4-Benzoyl-morpholino-cytosine-5'-O-phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides.
N-DMTr-N6-Benzoyl-morpholino-A-5'-O-phosphoramidite V80921 N-DMTr-N6-Benzoyl-morpholino-A-5'-O-phosphoramidite N-DMTr-N6-Benzoyl-morpholino-A-5'-O-phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides.
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