RNA synthesis, which is also called DNA transcription, is a highly selective process.Beyond RNA synthesis, RNA polymerase II transcription plays a more active role in the maturation, monitoring, and export of mRNA to the cytoplasm.
In order to fully repair the DNA after a single-strand break, DNA ligase constructs the final phosphodiester bond using the complementary strand of the double helix as a template.Substrates with RNA strands or mismatched base pairs near the ends of the nicked DNA are discriminated against by DNA ligases. Bleomycin (BLM) is a S-independent radiomimetic agent that damages DNA by creating free radicals that attack the C-4′ position in the deoxyribose backbone. This causes the ribose ring to open and strand breaks to occur.
M-MuLV Reverse Transcriptase (RNase H) and random hexamer primers are used to create first strand cDNA. The next step involves the use of DNA Polymerase I and RNase H to create second strand cDNA. Exonuclease/polymerase activity transforms the remaining overhangs into blunt ends. DNA fragments' 3′ ends are adenylated before being ligated with a NEBNext Adaptor with a hairpin loop structure to get the samples ready for hybridization. The two pathways that BET bromodomain inhibition most commonly affects are cell cycle and DNA replication. The translation of mRNA into proteins is inhibited by cycloheximide.
| Structure | Cat No. | Product Name | CAS No. | Product Description |
|---|---|---|---|---|
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V53037 | 5'-O-(4,4'-Dimethoxytrityl)-2'-deoxyuridine (5'-O-DMT-dU) | 23669-79-6 | 5'-O-(4,4'-Dimethoxytrityl)-2'-deoxyuridine (5'-O-DMT-dU) is a novel inhibitor of Escherichia coli dUTP nucleotidyl hydrolase (dUTPase), Ki value Above 1000 μM. 5'-O-(4,4'-Dimethoxytrityl)-2'-deoxyuridine synthesizes nucleoside phosphoramidite blocks, which are used in machine-assisted DNA synthesis. |
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V55170 | 5'-O-DMT-5-Ethynyl-2'-deoxyuridine 3'-CE phosphoramidite | 188411-06-5 | 5'-O-DMT-5-Ethynyl-2'-deoxyuridine 3'-CE phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides. |
|
V53031 | 5'-O-DMT-N2-ibu-dG (N2-Isobutyryl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyguanosine) | 68892-41-1 | 5'-O-DMT-N2-ibu-dG (N2-Isobutyryl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyguanosine) is a deoxynucleoside that can be used for oligonucleotides preparation. |
|
V53034 | 5'-O-DMT-N4-Ac-dC (N4-Acetyl-2'-deoxy-5'-O-DMT-cytidine) | 100898-63-3 | 5'-O-DMT-N4-Ac-dC (N4-Acetyl-2'-deoxy-5'-O-DMT-cytidine, compound 7), a deoxynucleoside with potential applications in the production of dodecylphosphorous amid (a raw material for the synthesis of amphiphilic DNA). |
|
V77666 | 5'-O-DMTr-2'-FU-methyl phosphonamidite | 5'-O-DMTr-2'-FU-methyl phosphonamidite, 5'-O-DMTr-2'-deoxy-2'-fluorouridine-3'-O-(P-methyl-N,N-diisopropylamino) phosphonamidite Yes A phosphoramidite monomer useful in solid-phase synthesis of oligonucleotides. | |
|
V77883 | 5'-O-DMTr-2'-OMe-5MeU-P-methyl phosphonamidite | 5'-O-DMTr-2'-OMe-5MeU-P-methyl phosphonamidite is a phosphoramidite monomer that may be utilized in the synthesis/preparation of oligonucleotides. | |
|
V77667 | 5'-O-DMTr-dU-methyl phosphonamidite | 5'-O-DMTr-dU-methyl phosphonamidite; 5'-O-DMTr-2'-deoxyuridine-3'-O-(P-methyl-N,N-diisopropylamino)phosphonamidite is a phosphoramidite monomer, Can be used for solid-phase synthesis of oligonucleotides. | |
|
V53215 | 5-Fluoro-2′-deoxy-UTP | 2710-64-7 | 5-Fluoro-2′-deoxy-UTP can be used as a substrate for DNA synthesis. |
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V55163 | 5-Fluoro-2′-deoxy-UTP sodium | 1630828-71-5 | 5-Fluoro-2′-deoxy-UTP sodium can be used as a substrate for DNA synthesis. |
|
V55167 | 5-Formylindole-CE phosphoramidite | 460355-05-9 | 5-Formylindole-CE phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides. |
|
V13781 | 5-Iminodaunorubicin (5-iminodaunorubicin) | 72983-78-9 | 5-Iminodaunorubicin is a quinone-modified anthracycline active molecule with anti-tumor activity. |
|
V15404 | 5-Iminodaunorubicin hydrochloride (5-iminodaunorubicin hydrochloride) | 67324-99-6 | 5-Iminodaunorubicin hydrochloride is a quinone-modified anthracycline active molecule with anti-tumor activity. |
|
V55176 | 5-Me-DMT-2'-O-Me-C(Bz)-CE-Phosphoramidite | 166593-57-3 | 5-Me-DMT-2'-O-Me-C(Bz)-CE-Phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides. |
|
V3120 | 5-methoxyflavone | 42079-78-7 | 5-methoxyflavone, a flavonoid analog, is a novel DNA polymerase-ß inhibitor and neuroprotective agent against ß-amyloid toxicity. |
|
V77663 | 5-Methylcytidine 5′-triphosphate trisodium (5-Methyl-CTP trisodium) | 5-Methylcytidine 5′-triphosphate (5-Methyl-CTP) trisodium is a modified nucleoside triphosphate. | |
|
V53225 | 5-PA-dUTP (5-Propargylamino-dUTP) | 179101-49-6 | 5-PA-dUTP (5-Propargylamino-dUTP) is a C5-modified nucleotide that can be incorporated into DNA nanoparticles (DNPs) for the delivery of photosensitizers. |
|
V55137 | 5-Propargylamino-3'-azidomethyl-dCTP | 666847-71-8 | 5-Propargylamino-3'-azidomethyl-dCTP is a nucleotide molecule that can be used in DNA synthesis and DNA sequencing. |
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V55147 | 5-Propargylamino-3'-azidomethyl-dUTP | 666847-57-0 | 5-Propargylamino-3'-azidomethyl-dUTP is a nucleotide molecule used in DNA synthesis and DNA sequencing. |
|
V53216 | 5-Propargylamino-dCTP | 115899-39-3 | 5-Propargylamino-dCTP is a nucleotide molecule that can be conjugated to molecular markers for nucleic acid labeling or sequence analysis. |
|
V55114 | 5-Propargylamino-ddCTP | 114748-56-0 | 5-Propargylamino-ddCTP is a nucleoside molecule that can be used to create nucleotide-cyanine dye conjugates for sequence analysis or nucleic acid labeling. |
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