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DNA(RNA) Synthesis

DNA(RNA) Synthesis

RNA synthesis, which is also called DNA transcription, is a highly selective process.Beyond RNA synthesis, RNA polymerase II transcription plays a more active role in the maturation, monitoring, and export of mRNA to the cytoplasm.

In order to fully repair the DNA after a single-strand break, DNA ligase constructs the final phosphodiester bond using the complementary strand of the double helix as a template.Substrates with RNA strands or mismatched base pairs near the ends of the nicked DNA are discriminated against by DNA ligases. Bleomycin (BLM) is a S-independent radiomimetic agent that damages DNA by creating free radicals that attack the C-4′ position in the deoxyribose backbone. This causes the ribose ring to open and strand breaks to occur.

M-MuLV Reverse Transcriptase (RNase H) and random hexamer primers are used to create first strand cDNA. The next step involves the use of DNA Polymerase I and RNase H to create second strand cDNA. Exonuclease/polymerase activity transforms the remaining overhangs into blunt ends. DNA fragments' 3′ ends are adenylated before being ligated with a NEBNext Adaptor with a hairpin loop structure to get the samples ready for hybridization. The two pathways that BET bromodomain inhibition most commonly affects are cell cycle and DNA replication. The translation of mRNA into proteins is inhibited by cycloheximide.

DNA(RNA) Synthesis related products

Structure Cat No. Product Name CAS No. Product Description
3′-Deoxyadenosine 5′-triphosphate V96796 3′-Deoxyadenosine 5′-triphosphate 73-04-1 3′-Deoxyadenosine 5′-triphosphate can be used in nucleic acid synthesis.
3′-Deoxyadenosine 5′-triphosphate trisodium V96784 3′-Deoxyadenosine 5′-triphosphate trisodium 71997-32-5 3′-deoxyadenosine 5′-triphosphate trisodium can be used in nucleic acid synthesis.
3′-Deoxythymidine V106576 3′-Deoxythymidine 3416-05-5 3′-Deoxythymidine (2′,3′-dideoxythymidine) is a nucleoside analog that inhibits eukaryotic and viral DNA polymerases and inhibits retroviral infection in certain cells.
3'-DMTr-dA V55201 3'-DMTr-dA 140712-82-9 3'-DMTr-dA is used for nucleotide synthesis.
3'-F-3'-dA(Bz)-2'-Phosphoramidite V55178 3'-F-3'-dA(Bz)-2'-Phosphoramidite 2127174-09-6 3'-F-3'-dA(Bz)-2'-Phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides.
3′-O-Azidomethyl-dCTP sodium V88658 3′-O-Azidomethyl-dCTP sodium 3′-O-Azidomethyl-dCTP sodium is a labeled deoxyoligonucleotide (dNTP) that can release pyrophosphate to generate fluorescence and has special applications in gene synthesis and sequencing.
3′-O-Azidomethyl-dTTP sodium V89130 3′-O-Azidomethyl-dTTP sodium 3′-O-Azidomethyl-dTTP sodium is a labeled deoxyoligonucleotide (dNTP) that can release pyrophosphate to generate fluorescence and has special applications in gene synthesis and sequencing.
3'-P-dTTP sodium V88660 3'-P-dTTP sodium 3'-P-dTTP sodium is a labeled modified deoxyoligonucleotide (dNTP) that can release pyrophosphate to generate fluorescence and has special applications in gene synthesis and sequencing.
3'-TBDMS-Bz-rA Phosphoramidite (3'-TBDMS-Bz-rA 磷酰胺) V55125 3'-TBDMS-Bz-rA Phosphoramidite (3'-TBDMS-Bz-rA Phosphoramidite) 129451-75-8 3'-TBDMS-Bz-rA Phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides.
3'-TBDMS-ibu-rG Phosphoramidite V55190 3'-TBDMS-ibu-rG Phosphoramidite 1445905-51-0 3'-TBDMS-ibu-rG Phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides.
3'-UMP disodium V102941 3'-UMP disodium 35170-03-7 3'-UMP disodium is a nucleotide, a component of ribonucleic acid (RNA), that is converted to uracil in the rat jejunum.
3'Ome-m7GpppAmpG ammonium V77323 3'Ome-m7GpppAmpG ammonium 3'Ome-m7GpppAmpG ammonium is a trinucleotide cap analog containing locked nucleic acid (LNA) molecules.
3,4-二羟基苯甲羟肟酸 V5043 Didox (NSC-324360) 69839-83-4 Didox (also known as NSC-324360) is a novel, potent and synthetic ribonucleotide reductase (RR) inhibitor.
3-Amino-4,6-dimethylpyridine V106492 3-Amino-4,6-dimethylpyridine 1193-71-1 3-Amino-4,6-dimethylpyridine (5A-DMP) is a UHRF1 inhibitor that targets the Arg-binding cavity of the UHRF1 TTD domain.
3-Cyanovinylcarbazole phosphoramidite V55152 3-Cyanovinylcarbazole phosphoramidite 1157899-72-3 3-Cyanovinylcarbazole phosphoramidite is a potent antiviral molecule that has the ability to stop the synthesis of viral DNA.
3-Hydroxy-2-methylpyridine (3-羟基-2-甲基吡啶) V53337 3-Hydroxy-2-methylpyridine 1121-25-1 3-Hydroxy-2-methylpyridine is extracted from theobromine extract and may be utilized in the preparation /synthesis of pyrimidines.
4-Ethylcatechol V92079 4-Ethylcatechol 1124-39-6 4-Ethylcatechol is a cyclic dihydroxylated metabolite of 4-Ethylphenol that can cause oxidative DNA damage.
4-Methoxyestradiol V55180 4-Methoxyestradiol 26788-23-8 4-Methoxyestradiol is methoxyestradiol that causes human lung epithelial cells to experience oxidative DNA damage.
4-Methoxyphenethyl alcohol (大茴香醇; p-Methoxyphenylethanol) V53010 4-Methoxyphenethyl alcohol (p-Methoxyphenylethanol) 702-23-8 4-Methoxyphenethyl alcohol, an aromatic alcohol, is the main component of the anise-like odor produced by Amorphophallus.
5'-Cholesteryl-TEG phosphoramidite V55177 5'-Cholesteryl-TEG phosphoramidite 1325147-19-0 5'-Cholesteryl-TEG phosphoramidite is a phosphoramidite monomer that may be utilized in the solid-phase synthesis of oligonucleotides.
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