| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
|
||
| 25mg |
|
||
| 50mg |
|
||
| 100mg |
|
||
| 250mg |
|
||
| 500mg |
|
||
| 1g |
|
||
| 2g |
|
||
| Other Sizes |
|
Purity: ≥98%
Propidium Iodide is a red fluorescent dye and DNA intercalating agent that can be used to stain cells. Propidium Iodide is used in flow cytometry as a DNA stain to evaluate cell viability or DNA content in cell cycle analysis, and in microscopy to visualise the nucleus and other DNA-containing organelles. Propidium Iodide cannot cross the membrane of live cells, making it useful to differentiate necrotic, apoptotic and healthy cells.
| Targets |
Nuclear staining agent
|
|---|---|
| ln Vitro |
Guidelines for use:
1.1 Preparation of stock solution: prepare 1 mg/mL stock solution in ddH2O. 1.2 Preparation of working solution: Dilute the Propidium Iodide stock solution to a working solution of 20–50 μg/mL using either preheated serum-free cell culture medium or PBS. Note: Before use, please make sure that the concentration of the propidium iodide working solution is appropriate for your specific needes, you may adjust the concentration ofworking solution and incubation period. 2. Cell staining (suspension cells) 2.1 Centrifuge the cells, add PBS, and wash twice for a total of five minutes. One x 10^6 cells per milliliter. 2.2 After adding 1 mL of working solution, let it sit at room temperature for five to ten minutes. 2.3 Centrifuge for 3–4 minutes at 400 g, then remove the supernatant. 2.4 Wash the cells twice, for five minutes each time, with PBS added. 2.5 After resuspending cells in 1 mL serum-free medium or PBS, observe using a fluorescence microscope or flow cytometer. 3. Cell staining (adherent cells) 3.1 On clean coverslips, cultivate the adhering cells. 3.2 Take out the coverslip and remove any extra culture medium. 3.3 Add 100 μL of the Propidium Iodide working solution, give the cells a brief shake to fully coat them, and let them sit for five to thirty minutes. Propidium Iodide. 3.4 Rinse with culture media after aspirating out the dye working solution. Use a fluorescent microscope to observe two to three times, for five minutes at a time. Note: If flow cytometry is to be used, the cells must first be digested by trypsin and resuspended before being stained. Storage conditions: -20°C, protect from light and being moisturized, 1 year. Safety notes: 1. Depending on the experimental needs, you may adjust the concentration of propidium iodide working solution and incubation period. 2. This product is intended solely for use in scientific research. It is prohibited from usage in food or medication, as well as in clinical diagnosis or treatment. 3. Propidium Iodide can cause cancer.Please wear a lab coat and disposable gloves for your health and safety. |
| Enzyme Assay |
Flow cytometric analysis: Propidium iodide is prepared in 0.1% sodium citrate + 0.1% Triton X-100 (50 μg/mL). Then the 200 ×g centrifuged cell pellet is gently resuspended in 1.5 mL hypotonlc fluorochrome solution (Propidium iodide 50 μg/mL), in 12×75 polypropylene tubes. The tubes are placed at 4°C in the dark overnight before the flow cytometric analysis. The propidium Iodide fluorescence of individual nuclei is measured using a FACScan flow cytometer. The nuclei traverses the light beam of a 488 nm Argon laser. A 560 nm dichrolc nurror (DM 570) and a 600 nm band pass filter (bandwidth 35 nm) are used for collecting the red fluorescence due to propidium Iodide staining of DNA and the data are registered on a logarithmic scale. [2]
|
| Cell Assay |
Propidium Iodide is a red fluorescent used to stain cells. Propidium Iodide only can be taken up by cells with impaired membrane integrity, it can be used in cell cycle and cell viability assays, as well necrocytosis assays.
Example 1, the method for cell nucleus staining is shown below: 1. Cells are washed with PBS, and then incubates cells with Propidium Iodide (15 min). 2. Fluorescent images are acquired with a confocal laser scanning microscope. Example 2, the method for cell staining is shown below: 1. Fix cultured cells with paraformaldehyde (4%; 10 min). 2. Incubate cells with Propidium Iodide (50 mg/L; 15 min; dark). 3. Fluorescent images are acquired with a confocal laser scanning microscope. |
| References |
[1]. Hezel M, et al. Propidium iodide staining: a new application in fluorescence microscopy for analysis of cytoarchitecture in adult and developing rodent brain. Micron. 2012 Oct;43(10):1031-8.
[2]. Nicoletti I, et al. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods. 1991 Jun 3;139(2):271-9. [3]. A rapid and simple method for measuring thymocyte apoptosis by propidium iodidestaining and flow cytometry. J Immunol Methods. 1991 Jun 3;139(2):271-9 |
| Molecular Formula |
C27H34I2N4
|
|---|---|
| Molecular Weight |
668.41
|
| Exact Mass |
668.08729
|
| Elemental Analysis |
C, 48.52 H, 5.13 I, 37.97 N, 8.38
|
| CAS # |
25535-16-4
|
| Appearance |
Pink to red solid powder
|
| tPSA |
55.9Ų
|
| SMILES |
C[N+](CC)(CCC[N+]1=C(C2=CC=CC=C2)C3=CC(N)=CC=C3C4=C1C=C(N)C=C4)CC.[I-].[I-]
|
| InChi Key |
XJMOSONTPMZWPB-UHFFFAOYSA-M
|
| InChi Code |
InChI=1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
|
| Chemical Name |
3-(3,8-Diamino-6-phenylphenanthridin-5-ium-5-yl)propyl-diethyl-methylazanium diiodide
|
| Synonyms |
Propidium Diodide Propidium Iodide
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~149.61 mM)
H2O : ~3.57 mg/mL (~5.34 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (3.74 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (3.74 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: Solubility in Formulation 1: ≥ 2.5 mg/mL (3.7 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.4961 mL | 7.4804 mL | 14.9609 mL | |
| 5 mM | 0.2992 mL | 1.4961 mL | 2.9922 mL | |
| 10 mM | 0.1496 mL | 0.7480 mL | 1.4961 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
| NCT05001386 | Not yet recruiting | Biological: Blood collection for the evaluation of the anti-drugs sensitivity |
Myeloproliferative Disorders Lymphoproliferative Disorders |
Hospices Civils de Lyon | March 2022 | Not Applicable |
| NCT06331676 | Not yet recruiting | Procedure: Tissue collection Other: Data collection |
Endometriosis | Hospices Civils de Lyon | April 2024 | Not Applicable |
| NCT00775606 | Terminated Has Results | Drug: Lopinavir 400 mg/ritonavir 100 mg Drug: Efavirenz |
Acquired Immune Deficiency Syndrome | Rush University Medical Center | October 2008 | Phase 4 |
| NCT06312150 | Recruiting | Other: Analysis of peripheral blood (control group) |
Tumor | Meyer Children's Hospital IRCCS | December 17, 2019 | Not Applicable |
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
COA