| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
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Purity: ≥98%
| ln Vitro |
The rhodamine 123 working solution is prepared. 1.1 Making the stock solution Take 1 milligram of rhodamine 123 and dissolve it in 525 μL DMSO to make a 5 mM stock solution. 1.2 Making the working solution for rhodamine 123 To create a working solution ranging from 1 to 20 μM, utilize serum-free cell culture medium or PBS stock solution. Note: The concentration of the Rhodamine 123 working solution should be adjusted based on the specific circumstances. 2. The staining of cells In a 6-well plate, 2.1 suspended cells a. Discard the supernatant after centrifuging at 1000 g for three to five minutes at 4°C. c. After adding 1 mL of the working solution, observe for one to three hours. c. Centrifuge at 400 g for three to four minutes at 4 °C; remove supernatant. d. Wash twice, for five minutes each time. One x 10^6 cells per milliliter. Wash for five minutes each time, twice, using PBS. e. Re-suspend cells in PBS or serum-free culture medium. either flow cytometry monitoring or fluorescence microscopy. 2.2 Adherent cells Adherent cells should be cultured on sterile glass slides (a). a. Take off the coverslip from the culture medium and use an aspirator to remove any extra. c. Add 100 μL working solution, give the cells a gentle shake to cover them fully, and leave them in place for 30 to 60 minutes. d. Use medium and wash twice in about five minutes. either flow cytometry monitoring or fluorescence microscopy. Note: Prior to staining, the cells must be resuspended if flow cytometry is being employed for detection.
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| References |
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| Additional Infomation |
A fluorescent probe with low toxicity which is a potent substrate for ATP BINDING CASSETTE TRANSPORTER, SUBFAMILY B, MEMBER 1 and the bacterial multidrug efflux transporter. It is used to assess mitochondrial bioenergetics in living cells and to measure the efflux activity of ATP BINDING CASSETTE TRANSPORTER, SUBFAMILY B, MEMBER 1 in both normal and malignant cells. (Leukemia 1997;11(7):1124-30)
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| Exact Mass |
380.092
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|---|---|
| CAS # |
62669-70-9
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| PubChem CID |
9929799
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| Appearance |
Pink to red solid powder
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| Melting Point |
235 °C
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| LogP |
5.535
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
27
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| Complexity |
706
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O1C2=C([H])/C(/C([H])=C([H])C2=C(C2=C([H])C([H])=C([H])C([H])=C2C(=O)OC([H])([H])[H])C2C([H])=C([H])C(=C([H])C1=2)N([H])[H])=N/[H]
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| InChi Key |
TUFFYSFVSYUHPA-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C21H17N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20/h2-11H,22-23H2,1H31H/q+1/p-1
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| Chemical Name |
Xanthylium, 3,6-diamino-9-(2-(methoxycarbonyl)phenyl)-, chloride
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| Synonyms |
Rhodamine 123 R 22420 R 302 RH 123.
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~62.5 mg/mL (~164.12 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
| NCT00972205 | COMPLETEDWITH RESULTS | Drug: paclitaxel Drug: CBT-1(Registered Trademark) Radiation: Tc 99m sestamibi |
Breast Cervical Lung Ovarian Renal |
National Cancer Institute (NCI) | 2007-12 | Not Applicable |
| NCT05206058 | COMPLETED | Procedure: venipuncture | Neutrophil Phyllanthus Abnormis Poisoning |
Chang Gung Memorial Hospital | 2022-01-01 |
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