| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
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| ln Vitro |
R-spondin-1 protein is introduced in high doses to begin and maintain organoids. In media lacking R-spondin-1, RS-246204, an R-spondin-1 substitute, stimulates the early development and proliferation of enteroids. Similar differentiation and self-renewal capacities are exhibited by intestinal cultures grown with RS-246204 and R-spondin-1. Moreover, organoid-based epithelial-mesenchymal transition models and forskolin-induced swelling can be effectively produced by enteroids derived from RS-246204 [1].
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| ln Vivo |
RS-246204 markedly elevated body weight and BrdU+ cells in the colitis model generated by DSS. These findings suggest that RS-246204 stimulates the R-spondin-dependent pathway in Lgr5 stem cells, which in turn promotes intestinal epithelial regeneration, lessens the severity of illness, and enhances proliferation or anti-apoptotic actions [1].
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| References |
| Molecular Formula |
C16H13N5O2S
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|---|---|
| Molecular Weight |
339.37
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| Exact Mass |
339.079
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| CAS # |
878451-87-7
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| PubChem CID |
6485821
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| Appearance |
White to light yellow solid powder
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| Density |
1.5±0.1 g/cm3
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| Index of Refraction |
1.784
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| LogP |
2.8
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
24
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| Complexity |
533
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
UHUHSGFUUWVRBO-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H13N5O2S/c1-20-12-13(21(2)15(23)19-14(12)22)18-16(20)24-11-8-7-9-5-3-4-6-10(9)17-11/h3-8H,1-2H3,(H,19,22,23)
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| Chemical Name |
3,7-dimethyl-8-quinolin-2-ylsulfanylpurine-2,6-dione
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| Synonyms |
RS246204; RS 246204; RS-246204
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~5 mg/mL (~14.73 mM)
H2O : < 0.1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 0.5 mg/mL (1.47 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 5.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 0.5 mg/mL (1.47 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 5.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9466 mL | 14.7332 mL | 29.4664 mL | |
| 5 mM | 0.5893 mL | 2.9466 mL | 5.8933 mL | |
| 10 mM | 0.2947 mL | 1.4733 mL | 2.9466 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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