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Sanguinarine chloride is a naturally occurring benzophenanthridine alkaloid isolated from the root of Sanguinaria Canadensis. It stimulates apoptosis via activating the production of reactive oxygen species (ROS).
| ln Vitro |
The activation of JNK and NF-κB signaling pathways is linked to apoptosis inducing sanguinarine (SANG). 22B-cFluc cells were stimulated with varying concentrations of sanguinarine for 24 hours in order to ascertain the impact of sanguinarine on cell viability. Following this, the CKK-8 test was carried out. The administration of sanguinarine exhibited a dose-dependent reduction in the proliferation of 22B cells. In parallel, cellular caspase-3 activity was assessed using the validated caspase-3 substrate Ac-DEVD-pNA in the cytoplasmic extracts of 22B-cFluc cells treated with various dosages of sanguinarine. Greater Sanguinarine-stimulated caspase-3 activity is indicated by a dose-dependent rise in absorbance at 450 nm [1].
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| ln Vivo |
In order to assess the in vivo apoptosis caused by sanguinarine (SANG), a xenograft model was created and one side of nude mice received a subcutaneous inoculation of 22B-cFluc cells. Intravenous sanguinarine (10 mg/kg) was administered to mice as a treatment. Mice were given intraperitoneal injections of 150 mg/kg D-luciferin substrate at 24, 48, and 72 hours post-treatment, followed by bioluminescence imaging. An important rise in luminescence signal was caused by sanguinarine administration as early as 48 hours following the start of treatment. Over the course of the experiment, bioluminescence imaging (BLI) intensity was seen to increase continuously. Tumors were collected 72 hours post-treatment, and apoptosis was evaluated using TUNEL labeling. Increased green signal of sporadic apoptotic cells suggested a considerable increase in apoptosis in the sanguinarine-treated group compared to control tumors [1].
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| References |
[1]. Wang Y, Noninvasive bioluminescence imaging of the dynamics of sanguinarine induced apoptosis via activation of reactive oxygen species. Oncotarget. 2016 Apr 19;7(16):22355-67
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| Molecular Formula |
C20H14CLNO4
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|---|---|
| Molecular Weight |
367.78
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| CAS # |
5578-73-4
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| Related CAS # |
Sanguinarine;2447-54-3
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| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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| SMILES |
[Cl-].O1C([H])([H])OC2C([H])=C([H])C3C(C1=2)=C([H])[N+](C([H])([H])[H])=C1C2=C([H])C4=C(C([H])=C2C([H])=C([H])C1=3)OC([H])([H])O4
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| Synonyms |
Sanguinarin chloride Sanguinarium chloride Pseudochelerythrine chloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~3.33 mg/mL (~9.05 mM)
H2O : < 0.1 mg/mL |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 0.33 mg/mL (0.90 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 3.3 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 0.33 mg/mL (0.90 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 3.3 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7190 mL | 13.5951 mL | 27.1902 mL | |
| 5 mM | 0.5438 mL | 2.7190 mL | 5.4380 mL | |
| 10 mM | 0.2719 mL | 1.3595 mL | 2.7190 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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