SC75741

Alias:
Cat No.:V0769 Purity: ≥98%
SC75741 (SC-75741; SC 75741) is a novel,selective and potent NF-κB inhibitor with potential antiviral and anti-inflammatory activity.
SC75741 Chemical Structure CAS No.: 913822-46-5
Product category: NF-κB
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
1mg
5mg
10mg
25mg
50mg
100mg
250mg
Other Sizes
Official Supplier of:
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text

 

  • Business Relationship with 5000+ Clients Globally
  • Major Universities, Research Institutions, Biotech & Pharma
  • Citations by Top Journals: Nature, Cell, Science, etc.
Top Publications Citing lnvivochem Products
Purity & Quality Control Documentation

Purity: ≥98%

Product Description

SC75741 (SC-75741; SC 75741) is a novel, selective and potent NF-κB inhibitor with potential antiviral and anti-inflammatory activity. With an EC50 of 200 nM, it blocks NF-κB. In non-toxic concentrations, SC75741 prevents influenza viruses (IV) from replicating. Reduced expression of cytokines, chemokines, and pro-apoptotic factors is the result of SC75741's impairment of NF-κB subunit p65's DNA binding. Following this, SC75741 prevents caspase activation and obstructs the nuclear export of viral ribonucleoproteins caused by caspase. The inhibitory potential of 5 uM SC75741 on virus replication in MDCK cells is greatest later in infection. SC75741 caused an effective, dose-dependent decline in viral titers in the human alveolar type II epithelial cell line A549 that was infected with the H5N1 and H7N7 viruses.

Biological Activity I Assay Protocols (From Reference)
Targets
NF-κB (EC50 = 200 nM)
ln Vitro
SC75741 exhibits immunosuppressive activity by limiting the proliferation of human PBMCs with IC50 of 2.2 μM.[1] SC75741 inhibits transcriptional levels of NF-κB mediated signaling, which prevents the replication of influenza A and B viruses. Additionally, SC75741 exhibits a high barrier to the emergence of virus variants with resistance.[2]
ln Vivo
SC75741 (15 mg/kg i.p.) reduces virus replication and cytokine expression in mice lungs after H5N1 influenza virus infection. Mice that have been exposed to SC75741 (intraperitoneal injection; 15 mg/kg; for 2 days) have 90% less H5N1 virus mRNA spread throughout their lungs.
The half-life of SC74751 is approximately 40 minutes, and its plasma levels (intravenously administered at 5 mg/kg and intraperitoneally administered at 15 mg/kg; for 3.5 and 6 hours) decrease mono-exponentially after intravenous administration. SC75741 has a half-life of 55 minutes after being administered intravenously, and it appears that this is due to a slow peritoneal uptake of the drug.
Enzyme Assay
In accordance with the manufacturer's instructions, the A549-NF-κB-SEAP cell line is used to prepare the NF-κB reporter gene assay. In essence, pNF-κB-SEAP reporter gene plasmid-stably transfected A549 cells are plated and given the night to attach. The cells are then stimulated with 10 ng/ml TNF-α for 22 hours after being incubated with the aforementioned compounds at concentrations of 100, 30, 10, 3, 1, 0.3, 0.1, and 0 μM for 5 hours. Using a chemiluminescent SEAP reporter gene assay, the cell's supernatant is examined for SEAP activity.
Cell Assay
Cell viability assay is prepared using a CellTiter-BluTM Cell Viability Assay. Four replicates are measured for each compound concentration.
Animal Protocol
Inbred female C57BL/6 mice at the age of 6-8 weeks[2]
15 mg/kg
Intraperitoneal injection; for 2 days
References

[1]. Bioorg Med Chem Lett . 2007 Nov 1;17(21):5858-62.

[2]. Cell Microbiol . 2013 Jul;15(7):1198-211.

These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C29H23N7O2S2
Molecular Weight
565.67
Exact Mass
565.14
Elemental Analysis
C, 61.58; H, 4.10; N, 17.33; O, 5.66; S, 11.34
CAS #
913822-46-5
Appearance
Solid powder
SMILES
C1CN(CCC1C2=NC(=CS2)C(=O)NC3=NC4=C(N3)C=C(C=C4)C(=O)C5=CC=CC=C5)C6=NC=NC7=C6SC=C7
InChi Key
QNZVBFMXWNWVKG-UHFFFAOYSA-N
InChi Code
InChI=1S/C29H23N7O2S2/c37-24(17-4-2-1-3-5-17)19-6-7-20-22(14-19)34-29(33-20)35-27(38)23-15-40-28(32-23)18-8-11-36(12-9-18)26-25-21(10-13-39-25)30-16-31-26/h1-7,10,13-16,18H,8-9,11-12H2,(H2,33,34,35,38)
Chemical Name
N-(6-benzoyl-1H-benzimidazol-2-yl)-2-(1-thieno[3,2-d]pyrimidin-4-ylpiperidin-4-yl)-1,3-thiazole-4-carboxamide
Synonyms

SC-75741; SC 75741; SC75741

HS Tariff Code
2934.99.9001
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: ~35 mg/mL (~61.9 mM)
Water: <1 mg/mL
Ethanol: <1 mg/mL
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 1.7678 mL 8.8391 mL 17.6782 mL
5 mM 0.3536 mL 1.7678 mL 3.5356 mL
10 mM 0.1768 mL 0.8839 mL 1.7678 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

Molarity Calculator allows you to calculate the mass, volume, and/or concentration required for a solution, as detailed below:

  • Calculate the Mass of a compound required to prepare a solution of known volume and concentration
  • Calculate the Volume of solution required to dissolve a compound of known mass to a desired concentration
  • Calculate the Concentration of a solution resulting from a known mass of compound in a specific volume
An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?
  • Enter 350.26 in the Molecular Weight (MW) box
  • Enter 10 in the Concentration box and choose the correct unit (mM)
  • Enter 5 in the Volume box and choose the correct unit (mL)
  • Click the “Calculate” button
  • The answer of 17.513 mg appears in the Mass box. In a similar way, you may calculate the volume and concentration.

Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:
  • Enter 10 into the Concentration (Start) box and choose the correct unit (mM)
  • Enter 25 into the Concentration (End) box and select the correct unit (mM)
  • Enter 25 into the Volume (End) box and choose the correct unit (mL)
  • Click the “Calculate” button
  • The answer of 62.5 μL (0.1 ml) appears in the Volume (Start) box
g/mol

Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
Instructions to calculate molar mass (molecular weight) of a chemical compound:
  • To calculate molar mass of a chemical compound, please enter the chemical/molecular formula and click the “Calculate’ button.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
  • Molecular mass (or molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
  • Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
/

Reconstitution Calculator allows you to calculate the volume of solvent required to reconstitute your vial.

  • Enter the mass of the reagent and the desired reconstitution concentration as well as the correct units
  • Click the “Calculate” button
  • The answer appears in the Volume (to add to vial) box
In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)
+
+
+

Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

Biological Data
  • SC75741
    SC75741 inhibits transcriptional activation of NF-κB-dependent genes.Cell Microbiol.2013 Jul;15(7):1198-211.
  • SC75741
    SC75741 reduces virus-induced expression of the NF-κB target genes IL-6 and TRAIL but not IFN-β or IRF-3 activity.Cell Microbiol.2013 Jul;15(7):1198-211.
  • SC75741
    SC75741 treatment influences cytokine expression in mice lungs after H5N1 influenza virus infection.Cell Microbiol.2013 Jul;15(7):1198-211.
Contact Us