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5mg |
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10mg |
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25mg |
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50mg |
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100mg |
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250mg |
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Purity: ≥98%
Schisandrin B (γ-Schisandrin; Sch B) is a naturally occuring and the most abundant dibenzocyclooctadiene lignan isolated from traditional Chinese medicinal herb Schisandra chinensis (Turcz.) with antioxidant effect on rodent liver and heart. Baill. It has been discovered that schisandrin B inhibits the in vitro lipid peroxidation of isolated rat liver microsomes by the NADPH/ascorbate and cysteine/ferric systems. Sch B was also demonstrated to scavenge superoxide and hydroxyl radicals in vitro using electron spin resonance measurements. Sch B could only deter the peroxidation reaction, in contrast to α-tocopherol'sprooxidantand antioxidant effect on Fe3+-induced lipid peroxidation.
Targets |
P-gp; ATR ( IC50 = 7.25 μM )
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ln Vitro |
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ln Vivo |
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Cell Assay |
Schisandrin B demonstrates anti-inflammatory properties by modifying the redox-sensitive transcription factors Nrf2 and NF-κB. The growth and cytokine release of lymphocytes induced by mitogens were suppressed by SB. In order to maintain cellular redox homeostasis and mitoenergetic capacity in neuronal cells, Sch B is thought to act as a hormetic agent and shield neuronal cells from oxidative stress. In microglia-neurons co-cultures, Sch B demonstrated strong neuroprotective effects against inflammatory damage mediated by microglia. Nitrite oxide (NO), prostaglandin E(2) (PGE(2)), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 are among the pro-inflammatory cytokines that Sch B markedly downregulated. Sch B has the ability to suppress TGF-β-induced epithelial-mesenchymal transition (EMT) in 4T1 cells and primary human breast cancer cells.
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Animal Protocol |
Mice endotoxic shock model
80 mg/kg Intraperitoneally injected (i.p.), single dose |
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References |
[1]. World J Gastroenterol . 2004 Oct 15;10(20):2944-8. [2]. Nucleic Acids Res . 2009 Sep;37(17):5678-89. [3]. Biochem Biophys Res Commun . 2005 Sep 23;335(2):406-11. [4]. lanta Med . 1995 Oct;61(5):398-401. [5]. Free Radic Biol Med . 1996;21(5):709-12. [6]. Cell Stress Chaperones . 2001 Jan;6(1):44-8. [7]. Biochem Pharmacol . 2006 Sep 28;72(7):824-37. [8]. WFitoterapia . 2011 Apr;82(3):393-400. [9]. PLoS One . 2012;7(7):e40480. [10]. Biochem Biophys Res Commun . 2005 Sep 23;335(2):406-11. |
Molecular Formula |
C23H28O6
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Molecular Weight |
400.46
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Exact Mass |
400.19
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CAS # |
61281-37-6
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Related CAS # |
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Appearance |
Powder
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SMILES |
CC1CC2=CC3=C(C(=C2C4=C(C(=C(C=C4CC1C)OC)OC)OC)OC)OCO3
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InChi Key |
RTZKSTLPRTWFEV-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C23H28O6/c1-12-7-14-9-16(24-3)20(25-4)22(26-5)18(14)19-15(8-13(12)2)10-17-21(23(19)27-6)29-11-28-17/h9-10,12-13H,7-8,11H2,1-6H3
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Chemical Name |
3,4,5,19-tetramethoxy-9,10-dimethyl-15,17-dioxatetracyclo[10.7.0.02,7.014,18]nonadeca-1(19),2,4,6,12,14(18)-hexaene
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Synonyms |
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.24 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.24 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (6.24 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.4971 mL | 12.4856 mL | 24.9713 mL | |
5 mM | 0.4994 mL | 2.4971 mL | 4.9943 mL | |
10 mM | 0.2497 mL | 1.2486 mL | 2.4971 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Sch B reduces 4T1 lung metastasis but does not affect the growth of primary tumor. PLoS One . 2012;7(7):e40480. td> |
Sch B reduces lung metastasis and prolongs mice survival time. PLoS One . 2012;7(7):e40480. td> |
Time course of Sch B pretreatment on TNFα-induced hepatic apoptosis and hepatocellular damage in mice: The effect of Sch B pretreatment. Cell Stress Chaperones . 2001 Jan;6(1):44-8. td> |
Time course of hepatic Hsp70 expression in Sch B–pretreated mice. Cell Stress Chaperones . 2001 Jan;6(1):44-8. td> |
Inhibition rate of Sch B on proliferation of SMMC-7721 cells. World J Gastroenterol . 2004 Oct 15;10(20):2944-8. td> |
Cytocidal effect of Sch B on growth of human endot-helial ECV-304 cells, human hepatic HL-7702 cells, primary human fibroblast cells and human lymphocyte cells. World J Gastroenterol . 2004 Oct 15;10(20):2944-8. td> |