| Size | Price | Stock | Qty |
|---|---|---|---|
| 25mg |
|
||
| 50mg |
|
||
| 100mg |
|
||
| 250mg |
|
||
| 500mg |
|
||
| Other Sizes |
|
| ln Vitro |
Treatment with Schisandra B (Gomisin-A; 1-10 μM; 2 days) decreases inflammatory markers associated with aging, including COX-2, IL1β, and TNF-α. Age-related β-galactosidase activity is decreased by schizandrol B [2]. Even in human diploid fibroblast (HDF) cells that have been exposed to stress-induced premature senescence (SIPS) [2], Schizandrol B (Gomisin-A; 1-10 μM; 2 days) suppresses the generation of reactive oxygen species. Schisandrol B (Gomisin-A; 1–10 μM) suppresses the translocation of NF-κB to the nucleus and the MAPK pathway [2]. By inhibiting the senescence process of SIPS-HDF cells, Schisandrol B (Gomisin-A; 1-10 μM) stimulates autophagy and mitochondrial biogenesis factors through the translocation of Nrf-2 [2]. Schisandrol B (0-80 μM) inhibits CYP2E1 and CYP3A11 activity, which drastically changes APAP metabolic activation [1].
|
|---|---|
| ln Vivo |
At a specific dosage, elevated and GSH depletion can be prevented, and the rise in alanine aminotransferase and aspartate aminotransferase activities can be considerably reduced by schisandra B (12.5–200 mg/kg; oral; 7 times, 12 hours apart) pretreatment. dependence approach. The activation of p53 and p21 caused by APAP is eliminated by Schisandrol B, and liver regeneration and anti-apoptosis-related proteins such BCL-2, PCNA, and cyclin D1 (CCND1) are expressed more frequently [1].
|
| Cell Assay |
Western Blot Analysis[2]
Cell Types: Human Diploid Fibroblasts (HDF) Tested Concentrations: 1 μM, 10 μM Incubation Duration: 3 days Experimental Results: Reduction of aging-related inflammatory molecules such as COX-2, IL1β, and TNF- α. |
| Animal Protocol |
Animal/Disease Models: Male C57BL/6 mice (6-8 weeks old, 20-22 g) were injected with acetaminophen (APAP) [1] Doses: 12.5 mg/kg, 12.5 mg/kg and 200 mg/kg administered Method: Oral administration; seven times, 12 hrs (hrs (hours)) apart.
Experimental Results: It has a protective effect on liver damage in mice caused by APAP. |
| References |
|
| Additional Infomation |
Besigomsin has been reported in Kadsura interior, Kadsura heteroclita, and Schisandra chinensis with data available.
See also: Schisandra chinensis fruit (part of). |
| Molecular Formula |
C23H28O7
|
|---|---|
| Molecular Weight |
416.4642
|
| Exact Mass |
416.183
|
| CAS # |
58546-54-6
|
| PubChem CID |
3001662
|
| Appearance |
White to off-white solid powder
|
| Density |
1.2±0.1 g/cm3
|
| Boiling Point |
579.7±50.0 °C at 760 mmHg
|
| Melting Point |
88.5°C
|
| Flash Point |
304.4±30.1 °C
|
| Vapour Pressure |
0.0±1.7 mmHg at 25°C
|
| Index of Refraction |
1.561
|
| LogP |
4.77
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
7
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
30
|
| Complexity |
588
|
| Defined Atom Stereocenter Count |
2
|
| SMILES |
C[C@H]1CC2=CC3=C(C(=C2C4=C(C(=C(C=C4C[C@]1(C)O)OC)OC)OC)OC)OCO3
|
| InChi Key |
ZWRRJEICIPUPHZ-MYODQAERSA-N
|
| InChi Code |
InChI=1S/C23H28O7/c1-12-7-13-8-16-20(30-11-29-16)22(28-6)17(13)18-14(10-23(12,2)24)9-15(25-3)19(26-4)21(18)27-5/h8-9,12,24H,7,10-11H2,1-6H3/t12-,23-/m0/s1
|
| Chemical Name |
(9S,10S)-3,4,5,19-tetramethoxy-9,10-dimethyl-15,17-dioxatetracyclo[10.7.0.02,7.014,18]nonadeca-1(19),2,4,6,12,14(18)-hexaen-9-ol
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~120.06 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.00 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (6.00 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (6.00 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4012 mL | 12.0060 mL | 24.0119 mL | |
| 5 mM | 0.4802 mL | 2.4012 mL | 4.8024 mL | |
| 10 mM | 0.2401 mL | 1.2006 mL | 2.4012 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
