Size | Price | |
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100mg | ||
250mg | ||
500mg |
ln Vitro |
At IC50 values of 3873 μM and 3722 μM, respectively, secnidazole (RP-14539) hemihydrate (0-5000 μM; 5 or 10 minutes) inhibits CYP2C19 and CYP3A4 [2]. Time-dependent inhibition is not observed with secnidazole hemihydrate (0-5000 μM; 5 or 10 minutes) [2]. Human ALDH2 is directly inhibited by senicidazole hemihydrate (0-5000 μM; 5 or 10 minutes), with an apparent IC50 of 503 μM [2]. At higher concentrations, secnidazole hemihydrate (0-5000 μM; 5 or 10 minutes) inhibits various CYP isomers, including CYP2A6, CYP2B6, and CYP2D6 [2]. Secnidazole hemihydrate (10 μL; 20 hours; serially dilute the secnidazole solution twice using Mueller-Hinton broth to generate dilutions from 80 to 0.3125 mg/mL) suppresses Salmonella marcescens growth (MIC50). Ten milligrams per milliliter is the value[3].
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ln Vivo |
In mice, secnidazole (RP-14539) hemihydrate (100 μL; intraperitoneal injection; for 5 days) has a protective effect against Salmonella marcescens pathogenesis and lessens it [3].
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Cell Assay |
Cell Viability Assay[3]
Cell Types: S.marcescens Tested Concentrations: 10 μL (Secnidazole solution was serially diluted two-fold using Mueller-Hinton broth to obtain dilutions from 80 to 0.3125 mg/mL) Incubation Duration: 20 h Experimental Results: 2 mg/mL (equivalent to 1/5 MIC) has no inhibitory effect on the growth of Serratia marcescens. |
Animal Protocol |
Animal/Disease Models: Female healthy albino mouse[3]
Doses: 100 μL Administration Doses: 100 μL; intellectual property. ; Results for 5 days: Dramatically diminished the ability of bacteria to kill mice. |
References |
[1]. Secnidazole. LiverTox: Clinical and Research Information on Drug-Induced Liver Injury, National Institute of Diabetes and Digestive and Kidney Diseases, 25 February 2020.
[2]. Helen S Pentikis, et al. In vitro metabolic profile and drug-drug interaction assessment of secnidazole, a high-dose 5-nitroimidazole antibiotic for the treatment of bacterial vaginosis. Pharmacol Res Perspect. 2020 Aug;8(4):e00634. [3]. Ahdab N Khayyat, et al. Secnidazole Is a Promising Imidazole Mitigator of Serratia marcescens Virulence. Microorganisms. 2021 Nov 11;9(11):2333. |
Molecular Formula |
C14H24N6O7
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Molecular Weight |
388.376362800598
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CAS # |
227622-73-3
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Related CAS # |
Secnidazole;3366-95-8
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
OC(C)CN1C(=CN=C1C)[N+](=O)[O-].OC(C)CN1C(=CN=C1C)[N+](=O)[O-].O
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.5748 mL | 12.8740 mL | 25.7480 mL | |
5 mM | 0.5150 mL | 2.5748 mL | 5.1496 mL | |
10 mM | 0.2575 mL | 1.2874 mL | 2.5748 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.