Size | Price | Stock | Qty |
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5mg |
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10mg |
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25mg |
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50mg |
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100mg |
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Other Sizes |
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ln Vitro |
Semax (100 nM) has been shown to increase the survival of cholinergic basal forebrain neurons [3], decrease Abeta oligomer (100 μM) levels [2], stimulate choline acetyltransferase activity in isolated basal forebrain tissue cultures [3], and stimulate the synthesis of brain-derived neurotrophic factor (BDNF) in astrocytes cultured in rat basal forebrain [4]. Semax (25 μM and 100 μM, 48 hours) exhibits these effects [2], [3], and [4].
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ln Vivo |
Semax (100 μg/kg, intraperitoneal injection) increases the development and function of the vasculature in ischemic (pMCAO) rats [1]. Semax (50 µg and 250 µg, 100 µL/kg, intranasal inhalation) elevates b BDNF protein levels in the basal forebrain of rats [5].
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Animal Protocol |
Animal/Disease Models: ischemia (pMCAO) rat [1].
Doses: 100 μg/kg Route of Administration: intraperitoneal (ip) injection at 15 minutes, 1, 4 and 8 hrs (hrs (hours)) after permanent middle cerebral artery occlusion (pMCAO) Experimental Results: Enhanced immune response (transcript upregulation). Animal/Disease Models: Rat[5] Doses: 50 µg, 250 µg, 100 µL/kg Route of Administration: Intranasal inhalation Experimental Results: Brain-derived neurotrophic factor (BDNF) protein levels increased in the basal forebrain of rats. |
References |
[1]. Medvedeva EV, et al. The peptide semax affects the expression of genes related to the immune and vascular systems in rat brain focal ischemia: genome-wide transcriptional analysis. BMC Genomics. 2014 Mar 24;15:228.
[2]. Sciacca MFM, et al. Semax, a Synthetic Regulatory Peptide, Affects Copper-Induced Abeta Aggregation and Amyloid Formation in Artificial Membrane Models. ACS Chem Neurosci. 2022 Feb 16;13(4):486-496. [3]. Grivennikov IA, et al. Effects of behaviorally active ACTH (4-10) analogue - Semax on rat basal forebrain cholinergic neurons. Restor Neurol Neurosci. 2008;26(1):35-43. [4]. Shadrina MI, et al. Rapid induction of neurotrophin mRNAs in rat glial cell cultures by Semax, an adrenocorticotropic hormone analog. Neurosci Lett. 2001 Aug 3;308(2):115-8. [5]. Dolotov OV, et al. Semax, an analogue of adrenocorticotropin (4-10), binds specifically and increases levels of brain-derived neurotrophic factor protein in rat basal forebrain. J Neurochem. 2006 Apr;97 Suppl 1:82-6. |
Molecular Formula |
C37H51N9O10S
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Molecular Weight |
813.920147180557
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CAS # |
80714-61-0
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Related CAS # |
Semax acetate;2828433-33-4
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
CSCCC(N)/C(/O)=N/C(CCC(O)=O)/C(/O)=N/C(CC1=CN=CN1)/C(/O)=N/C(CC1=CC=CC=C1)C(N1C(/C(/O)=N/CC(N2C(C(O)=O)CCC2)=O)CCC1)=O
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.2286 mL | 6.1431 mL | 12.2862 mL | |
5 mM | 0.2457 mL | 1.2286 mL | 2.4572 mL | |
10 mM | 0.1229 mL | 0.6143 mL | 1.2286 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.