Size | Price | Stock | Qty |
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250mg |
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500mg |
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Other Sizes |
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ln Vitro |
Silymarin (0-120 μg/mL; 24 hours) suppresses the viability of AGS cells. At 20 μg/mL, 71.5% at 40 μg/mL, and 59.8% at 60 μg/mL, AGS cell viability is measured. At 80 μg/mL, 44.5%, 35.3%, and 33.9% were found [1]. At dilution, silymarin (40–80 μg/mL; 24 hours) inhibits AGS cells. At 40 μg/mL and 80 μg/mL, it inhibits AGS cell migration by 59.4% and 21.7%, respectively [1].
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ln Vivo |
During the forced swim test (FST), silymarin (oral gavage; 10, 20, 50, 100, and 200 mg/kg) shortens the stopping time in a dance pattern. Additionally, it lowers silymarin's ED50 in the tail suspension test (TST), which is roughly 10 mg/kg. In both trials, a dose of 100 mg/kg was found to be the most efficacious dose [3].
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Cell Assay |
Cell Viability Assay[2]
Cell Types: AGS cells Tested Concentrations: 20 µg/ml, migration of 40 µg. /ml, 80 µg/ml, 100 µg/ml and 120 µg/ml Incubation Duration: 24 hrs (hours) Experimental Results: Demonstrated significant concentration-dependent inhibitory effect on AGS cells starting from 20 µg/ml. |
References |
[1]. Féher J, et al. Silymarin in the prevention and treatment of liver diseases and primary liver cancer. Curr Pharm Biotechnol. 2012 Jan;13(1):210-7.
[2]. Sung-Hyun Kim, et al.Silymarin induces inhibition of growth and apoptosis through modulation of the MAPK signaling pathway in AGS human gastric cancer cells. Oncol Rep. 2019 Nov;42(5):1904-1914. [3]. Mina Khoshnoodi, et al. Possible involvement of nitric oxide in antidepressant-like effect of silymarin in male mice. Pharm Biol. 2015 May;53(5):739-45. [4]. Anna Maria Sardanelli, et al. SARS-CoV-2 Main Protease Active Site Ligands in the Human Metabolome. Molecules 2021, 26(5), 1409. |
Molecular Formula |
C25H22O10
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Molecular Weight |
482.44
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Exact Mass |
482.1213
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CAS # |
65666-07-1
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Related CAS # |
Silybin A;22888-70-6;Silybin B;142797-34-0
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
COC1C(O)=CC=C(C2OC3=CC(C4OC5=CC(=CC(O)=C5C(=O)C4O)O)=CC=C3OC2CO)C=1
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Synonyms |
Legalon 70; Milk thistle; Silymarin
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~100 mg/mL
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 3 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 30.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.0728 mL | 10.3640 mL | 20.7280 mL | |
5 mM | 0.4146 mL | 2.0728 mL | 4.1456 mL | |
10 mM | 0.2073 mL | 1.0364 mL | 2.0728 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.