| Size | Price | Stock | Qty |
|---|---|---|---|
| 25mg |
|
||
| 50mg |
|
||
| 100mg |
|
||
| 250mg |
|
||
| 500mg |
|
||
| Other Sizes |
|
| Targets |
NF-κB; MMP-9; p38 MAP kinase
|
|---|---|
| ln Vitro |
In the human colon derived crypt like HT-29 and Caco-2 epithelial cell lines, SM-7368 (5 μM) targets downstream of MAPK p38 activation[1].
SM-7368 inhibits TNF--α-induced MMP-9 upregulation in a concentration-dependent manner, reaching its peak activity at 10 μM. MMP-9 mRNA transcript accumulation and protein expression are suppressed by SM-7368 in response to TNFα. TNF-α induced NF-κB activity is severely inhibited by SM-7368, but AP-1 activity is not affected. The TNF-induced invasion of the human fibrosarcoma cell line HT1080 is severely inhibited by SM-7368[2].
TNF-α (20 ng/mL)-induced MMP-9 upregulation is significantly inhibited by SM-7368 (10-25 μM） This upregulation is almost entirely eliminated by 10 μM of SM-7368[2].
|
| References |
|
| Molecular Formula |
C10H5CLN4O5S
|
|---|---|
| Molecular Weight |
328.68
|
| Exact Mass |
327.967
|
| Elemental Analysis |
C, 36.54; H, 1.53; Cl, 10.79; N, 17.05; O, 24.34; S, 9.75
|
| CAS # |
380623-76-7
|
| Related CAS # |
380623-76-7
|
| PubChem CID |
11537217
|
| Appearance |
Off-white to light yellow solid powder
|
| LogP |
4.295
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
7
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
21
|
| Complexity |
442
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O=[N+](C1=CN=C(NC(C2C=CC([N+]([O-])=O)=C(Cl)C=2)=O)S1)[O-]
|
| InChi Key |
XCHLNGBTHLJLFG-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C10H5ClN4O5S/c11-6-3-5(1-2-7(6)14(17)18)9(16)13-10-12-4-8(21-10)15(19)20/h1-4H,(H,12,13,16)
|
| Chemical Name |
3-chloro-4-nitro-N-(5-nitro-1,3-thiazol-2-yl)benzamide
|
| Synonyms |
Activation Inhibitor III; SM-7368; SM7368; SM 7368;NF-kappaB
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 66~83.33 mg/mL (200.8~253.52 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.33 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0425 mL | 15.2124 mL | 30.4247 mL | |
| 5 mM | 0.6085 mL | 3.0425 mL | 6.0849 mL | |
| 10 mM | 0.3042 mL | 1.5212 mL | 3.0425 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
