Sodium salicylate

Alias:
Cat No.:V0767 Purity: ≥98%
Sodium salicylate (Magsalyl;Kerasalicyl; Kerosal), thesodium salt of salicylic acid and a metabolite of acetylsalicylic acid, is an inhibitor of NF-kB with potential anti-inflammatory activity.
Sodium salicylate Chemical Structure CAS No.: 54-21-7
Product category: NF-κB
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
10g
25g
50g
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Other Forms of Sodium salicylate:

  • Salicylic acid
Official Supplier of:
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Purity & Quality Control Documentation

Purity: ≥98%

Product Description

Sodium salicylate (Magsalyl; Kerasalicyl; Kerosal), the sodium salt of salicylic acid and a metabolite of acetylsalicylic acid, is an inhibitor of NF-kB with potential anti-inflammatory activity. It has been used as a nonsteroidal anti-inflammatory drug (NSAID). It works by permanently acetylating COX I/II (cyclooxygenases I and II), which prevents the production of prostaglandins, which is a key factor in inflammation and pain.

Biological Activity I Assay Protocols (From Reference)
Targets
COX-2; Microbial Metabolite; Autophagy; S6K
ln Vitro
At concentrations much lower than those necessary to prevent the activation of NF-κB (20 mg/mL), sodium salicylate effectively inhibits COX-2 activity. When combined with interleukin 1β for 24 hours, sodium salicylate inhibits prostaglandin E2 release with an IC50 value of 5 μg/mL, an effect that is not influenced by NF-κB activation, COX-2 transcription, or COX-2 translation. Additionally, sodium salicylate acutely (30 min) inhibits COX-2 activity as measured in the presence of 0, 1, or 10 μM exogenous arachidonic acid in a concentration-dependent manner. In contrast, sodium salicylate is a very weak inhibitor of COX-2 activity with an IC50 of >100 μg/mL when exogenous arachidonic acid is increased to 30 μM. With an apparent IC50 value of about 5 μg/mL when combined with IL-1 for 24 hours, sodium salicylate inhibits PGE2 release in a concentration-dependent manner. After a 30-min exposure period, different concentrations of exogenous arachidonic acid (1, 10, and 30 μM) are added, and then the ability of sodium salicylate to directly inhibit COX-2 activity in A549 cells is tested. In the absence of additional arachidonic acid or in the presence of 1 or 10 μM exogenous substrate, sodium salicylate inhibits COX-2 activity in a concentration-dependent manner, with an apparent IC50 value of roughly 5 μg/mL. With an apparent IC50 value of more than 100 g/mL and a maximal inhibition of less than 50%, sodium salicylate is an ineffective inhibitor of COX-2 activity when the same experiments are carried out using 30 μM arachidonic acid[1].
ln Vivo
Salicylate lowers plasma glucose levels in C57Bl/6 DIO mice during fasting and after meals. Additionally, after Salicylate treatment in C57Bl/6 DIO mice, there is a tendency to decrease plasma triglyceride levels (P=0.059). Salicylate significantly lowers 11β-HSD1 mRNA in the omental adipose tissue of C57Bl/6 DIO mice, with a trend toward the same result in the mesenteric adipose (P=0.057). Salicylate also lowers 11β-HSD1 enzyme activity in the mesenteric adipose of C57Bl/6 DIO mice[2].
Enzyme Assay
The cofactors Glutathione (5 mM), Adrenaline (5 mM), and Hematin (1 M) are dissolved in 50 mM Tris buffer (pH 7.5), along with human purified COX-2. Prior to further diluting in Tris buffer, hematin is first dissolved in a concentrated stock of 100 mM in 1 M NaOH. 96-well plates with individual wells are used to conduct enzyme reactions, with a final reaction volume of 200 μL. The plate is first filled with various sodium salicylate concentrations, then 10 enzyme units (180 μL) are added. Arachidonic acid (10 nM to 30 μM) is then added to the plates and incubated at 37 ° for an additional 15 min. The reaction is stopped by heating the plate to 100°C for five minutes. The 96-well plate is then centrifuged at 10,000 g for 10 min, after which the appropriate samples are taken out and added to the radioimmunoassay[1].
Cell Assay
A549 cells are first treated with IL-1β for 24 hours, and the culture medium is replaced with DMEM containing different concentrations of sodium salicylate (10, 100, and 1000 μg/mL) in order to assess the direct effect of sodium salicylate on COX-2 activity after induction. For 30 minutes, cells are incubated at 37°C. Following a 15-minute addition of arachide acid (1–30 μM), the medium is removed to measure PGE2[1].
Animal Protocol
Mice: Male C57Bl/6 adult mice are 12 weeks old. Prior to treatment, 10 weeks of a high-fat diet (58% fat, 12% sucrose) are given to C57Bl/6 mice that have developed diet-induced obesity (C57Bl/6 DIO). To groups of n=8, osmotic minipumps implanted subcutaneously between the scapulae are used to administer sodium salicylate (120 mg/kg/day) or distilled water (vehicle) starting one week after arrival (C57Bl/6 Lean), after ten weeks of high-fat feeding (C57Bl/6 DIO), or after reaching the target weight (HSD1KO-DIO) for four weeks.
References

[1]. Sodium salicylate inhibits cyclo-oxygenase-2 activity independently of transcription factor (nuclear factor kappaB) activation: role of arachidonic acid. Mol Pharmacol. 1997 Jun;51(6):907-12.

[2]. Palmitic acid negatively regulates tumor suppressor PTEN through T366 phosphorylation and protein degradation. Cancer Lett. 2020 Oct 8;S0304-3835(20)30506-1.

[3]. Salicylate downregulates 11β-HSD1 expression in adipose tissue in obese mice and in humans, mediating insulin sensitization. Diabetes. 2012 Apr;61(4):790-6.

[4]. Age-associated NF-κB signaling in myofibers alters the satellite cell niche and re-strains muscle stem cell function. Aging (Albany NY). 2016 Nov; 8(11): 2871–2884.

These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C7H6O3.NA
Molecular Weight
161.11
Exact Mass
160.01363830
Elemental Analysis
C, 52.51; H, 3.15; Na, 14.36; O, 29.98
CAS #
54-21-7
Appearance
Solid powder
SMILES
C1=CC=C(C(=C1)C(=O)[O-])O.[Na+]
InChi Key
ABBQHOQBGMUPJH-UHFFFAOYSA-M
InChi Code
InChI=1S/C7H6O3.Na/c8-6-4-2-1-3-5(6)7(9)10;/h1-4,8H,(H,9,10);/q;+1/p-1
Chemical Name
sodium;2-hydroxybenzoate
Synonyms

Sodium salicylate; Magsalyl; Kerasalicyl; Kerosal

HS Tariff Code
2934.99.03.00
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: ~32 mg/mL (~198.6 mM)
Water: ~32 mg/mL (~198.6 mM)
Ethanol: <1 mg/mL
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 6.2069 mL 31.0347 mL 62.0694 mL
5 mM 1.2414 mL 6.2069 mL 12.4139 mL
10 mM 0.6207 mL 3.1035 mL 6.2069 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

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Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
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Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
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Clinical Trial Information
NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05069740 Recruiting Drug: Salsalate Pill
Drug: Placebo
Endometriosis Penn State University January 1, 2022 Early Phase 1
NCT03229408 Recruiting Drug: Salsalate
Other: Placebo
Polycystic Ovary Syndrome University of Illinois at
Chicago
December 5, 2018 Phase 2
NCT01480297 Completed Drug: Salsalate Type 1 Diabetes
Peripheral Neuropathy
University of Michigan November 2011 Phase 2
NCT01182727 Completed Drug: salsalate Schizophrenia
Insulin Resistance
University of Maryland,
Baltimore
August 2010 Not Applicable
NCT01046682 Completed Drug: Salsalate HIV
Inflammation
University Hospitals
Cleveland Medical Center
January 2009 Phase 2
Biological Data
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