| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
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| ln Vitro |
Sophoridine (0-500 μM; 48 hours) has an IC50 value of roughly 20 μM to 200 μM, which significantly inhibits the growth of human pancreatic cancer, gastric cancer, liver cancer, colon cancer, gallbladder cancer, and prostate cancer cells [1]. Sophoridine (0-20 μM; 48 hours) increased the S-phase cell population in Miapaca-2 cells from 26.23% (control) to 38.67% and in PANC-1 cells from 29.56% (control). control) increased to 39.16%, approximately 1.5 times and 1.3 times respectively [1]. In contrast, sophoridin (0–20 μM; 48 hours) significantly lowers bcl-2 and bcl–xl levels while significantly raising the Bax/Bcl-2 ratio [1]. It also significantly raises bad and bax levels.
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|---|---|
| ln Vivo |
Sophoridine (ip; 20 or 40 mg/kg; 21 days) suppresses the growth of pancreatic tumors that have been xenografted [1].
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| Cell Assay |
Cell viability assay [1]
Cell Types: Normal cells: IOSE144, HL-7702 and LO2, BEAS-2B, GES-1, HEK 293 T, HPDE, FHC, human cancer cells: PANC-1, Mapaca-1, hepG2, SGC-7901, CBC-SD, SGC-996, PC-3, MKN-45, MGC-803, Hela and HCT116 cell Tested Concentrations: 0, 3.9, 7.8, 15.5, 31, 62.5, 125, 250, 500 μM Incubation Duration: 48 hrs (hours) Experimental Results: demonstrated the most effective cytotoxicity against cancer cells. Cell cycle analysis [1] Cell Types: PANC-1 cells; Miapca-2 cells Tested Concentrations: 20 μM Incubation Duration: 48 hrs (hours) Experimental Results: Resulting in S phase population accumulation. Western Blot Analysis [1] Cell Types: PANC-1 cells; Miapca-2 cells Tested Concentrations: 20 μM Incubation Duration: 48 hrs (hours) Experimental Results: Induction of activation of intrinsic apoptotic pathway. |
| Animal Protocol |
Animal/Disease Models: BALB/c homozygous (nu/nu) nude mice [1]
Doses: 20 or 40 mg/kg Route of Administration: intraperitoneal (ip) injection; 20 or 40 mg/kg; 21-day Experimental Results: Xenograft pancreatic tumor mass reduction. |
| References |
[1]. Xu Z, et al. Sophoridine induces apoptosis and S phase arrest via ROS-dependent JNK and ERK activation in human pancreatic cancer cells. J Exp Clin Cancer Res. 2017 Sep 11;36(1):124.
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| Molecular Formula |
C15H24N2O
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|---|---|
| Molecular Weight |
248.37
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| CAS # |
6882-68-4
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| Related CAS # |
d-Sophoridine;83148-91-8
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| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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| SMILES |
O=C1C([H])([H])C([H])([H])C([H])([H])[C@]2([H])[C@@]3([H])C([H])([H])C([H])([H])C([H])([H])N4C([H])([H])C([H])([H])C([H])([H])[C@]([H])(C([H])([H])N21)[C@]43[H]
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~201.32 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.17 mg/mL (8.74 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 21.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.17 mg/mL (8.74 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 21.7 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.17 mg/mL (8.74 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.0263 mL | 20.1313 mL | 40.2625 mL | |
| 5 mM | 0.8053 mL | 4.0263 mL | 8.0525 mL | |
| 10 mM | 0.4026 mL | 2.0131 mL | 4.0263 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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