Size | Price | Stock | Qty |
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1mg |
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5mg |
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10mg |
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Other Sizes |
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ln Vitro |
SPOP-IN-6b has an IC50 of 2-10.2 μM against A498, Caki-2, Ketr-3, 769-P, 0S-RC-2, and 786-0 cells [1]. By inhibiting SPOP activity, SPOP-IN-6b (0.1-3.0 μM; 10 hours) can increase the amount of the inhibitory cancer substrate proteins PTEN and DUSP7 [1].
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ln Vivo |
By suppressing SPOP, SPOP-IN-6b (40–80 mg/kg; intraperitoneal injection; daily; for 25 days) can limit the growth of tumors [1].
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Animal Protocol |
Animal/Disease Models: Nude mice [1]
Doses: 40 mg/kg, 60 mg/kg, 80 mg/kg Route of Administration: intraperitoneal (ip) injection; daily; 25-day Experimental Results: Slowed tumor growth by inhibiting SPOP. |
References |
[1]. Guangcai Y, et al. The pyrido-pyrimidines of 2- imines -5- ketone group -2,5- dihydros -1-H- two. CN107141287A.
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Molecular Formula |
C28H32N6O3
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Molecular Weight |
500.6
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Exact Mass |
500.2536
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CAS # |
2136270-20-5
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Related CAS # |
SPOP-IN-6b dihydrochloride;SPOP-IN-6b hydrochloride;2136270-68-1
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
O1CCN(CC1)CCCNC(C1=CC2C(N3C=CC=C(C)C3=NC=2N(C1=N)CCC1C=CC=CC=1)=O)=O
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Synonyms |
SPOP-IN-6b; SPOP IN 6b; SPOP IN-6b
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~10 mg/mL (~19.98 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1 mg/mL (2.00 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1 mg/mL (2.00 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.9976 mL | 9.9880 mL | 19.9760 mL | |
5 mM | 0.3995 mL | 1.9976 mL | 3.9952 mL | |
10 mM | 0.1998 mL | 0.9988 mL | 1.9976 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.