| Size | Price | Stock | Qty |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
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| ln Vitro |
In INS-1 cells, SRI-37330 (1 μM, 24 hours) suppresses the activity of the human TXNIP promoter [1]. In INS-1 cells, TXNIP's mRNA and protein levels are inhibited by SRI-37330 (1 μM, 24 hours) [1]. The binding of polymerase II (Pol II) to the TXNIP promoter's E-box motif region is inhibited by SRI-37330 (5 μM, 24 hours) [1]. In TC1-6 cells, SRI-37330 (5 μM, 24 hours) decreases glucagon secretion [1]. In primary hepatocytes, glucagon-induced glucose production is inhibited by SRI-37330 (0-5 μM, 24 hours) [1].
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| ln Vivo |
SRI-37330 inhibits hepatic glucose production and decreases glucagon secretion when taken orally for three weeks at a dose of 100 mg/kg in drinking water [1]. Male C57BL/6J mice responded favorably to SRI-37330 (100 mg/kg, orally, in drinking water) when given for three weeks [1]. Mice treated with SRI-37330 (100 mg/kg, orally, in drinking water) for three weeks show improvement in hepatic steatosis, hyperglycemia caused by STZ, and obesity [1].
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| Cell Assay |
RT-PCR[1]
Cell Types: INS-1 Cell Tested Concentrations: 1 μM Incubation Duration: 24 h Experimental Results: Inhibits endogenous TXNIP mRNA expression with an IC50 of 0.64 μM. |
| Animal Protocol |
Animal/Disease Models: C57BL/6J mice[1]
Doses: 100 mg/kg Route of Administration: Orally (po), dissolved in drinking water, for 3 weeks. Experimental Results: diminished serum glucagon levels, inhibited hepatic glucose production and improved glucose homeostasis in mice. |
| References |
| Molecular Formula |
C16H19F3N4O2S
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|---|---|
| Molecular Weight |
388.41
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| Exact Mass |
388.12
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| Elemental Analysis |
C, 49.48; H, 4.93; F, 14.67; N, 14.42; O, 8.24; S, 8.25
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| CAS # |
2322245-42-9
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| Related CAS # |
SRI-37330 hydrochloride;2322245-49-6
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| PubChem CID |
142582737
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| Appearance |
White to off-white solid powder
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| LogP |
2.7
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
26
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| Complexity |
581
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
WRSNFEVXRKOMLL-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H19F3N4O2S/c1-26(24,25)22-8-11-3-2-6-23(9-11)15-13-7-12(16(17,18)19)4-5-14(13)20-10-21-15/h4-5,7,10-11,22H,2-3,6,8-9H2,1H3
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| Chemical Name |
N-[[1-[6-(trifluoromethyl)quinazolin-4-yl]piperidin-3-yl]methyl]methanesulfonamide
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| Synonyms |
SRI-37330 free base; SRI37330 free base, SRI 37330 free base
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~257.46 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5746 mL | 12.8730 mL | 25.7460 mL | |
| 5 mM | 0.5149 mL | 2.5746 mL | 5.1492 mL | |
| 10 mM | 0.2575 mL | 1.2873 mL | 2.5746 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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