| Size | Price | Stock | Qty |
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| 100mg |
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| 250mg |
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| 500mg |
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| 1g |
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| 2g |
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| Other Sizes |
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Purity: ≥98%
Sulforaphane (BroccoPhane; Detoxophane) is a naturally-occurring isothiocyanate widely found in consumed vegetables. As the aglycone metabolite of glucosinolate glucoraphanin (sulforaphane glucosinolate), sulforaphane acts as an antioxidant and potent stimulator of endogenous detoxifying enzymes. Sulforaphane activates Nrf2 and inhibits high glucose-induced progression of pancreatic cancer via AMPK dependent signaling. Sulforaphane has shown anti-cancer and anti-inflammatory activities.
| ln Vitro |
In a dose-dependent manner, sulforaphane causes cell cycle arrest and eventual cell death. Higher expression of cyclin A and B1 was linked to this sulforaphane-induced cell cycle halt. Sulforaphane causes apoptosis, which is the process by which cells die. Sulforaphane is less harmful to differentiated CaCo2 cells and slows the return of growth in quiescent colon cancer cells (HT29), while also reducing their cell viability [1]. Sulforaphane pretreatment of H9c2 rat myoblasts decreased the amount of apoptotic cells and pro-apoptotic protein expression (Bax, caspase-3, and cytochrome c), as well as the increase in mitochondrial membrane potential that doxorubicin caused. Furthermore, sulforaphane decreases doxorubicin-induced levels of reactive oxygen species (ROS, as determined by MitoSOX Red reagent) in mitochondria via increasing the mRNA and protein expression of heme oxygenase-1 [2].
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| ln Vivo |
Sulforaphane reduces mammary tumor growth in Sprague-Dawley rats treated with a single dose of 9,10-dimethyl-1,2-benzanthracene. Administration of sulforaphane lowers the incidence, multiplicity, and weight of breast tumors generated by a single dose of DMBA in female Sprague-Dawley rats and slows their progression [3].
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| References |
[1]. Gamet-Payrastre L, et al. Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in HT29 human colon cancer cells. Cancer Res. 2000 Mar 1;60(5):1426-33.
[2]. Li B, et al. Sulforaphane prevents doxorubicin-induced oxidative stress and cell death in rat H9c2 cells. Int J Mol Med. 2015 Jul;36(1):53-64. [3]. Zhang Y, et al. Anticarcinogenic activities of sulforaphane and structurally related synthetic norbornylisothiocyanates. Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3147-50. [4]. Chen X, et al. Activation of Nrf2 by Sulforaphane Inhibits High Glucose-Induced Progression of PancreaticCancer via AMPK Dependent Signaling. ell Physiol Biochem. 2018;50(3):1201-1215 |
| Molecular Formula |
C6H11NOS2
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|---|---|
| Molecular Weight |
177.3
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| CAS # |
4478-93-7
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| Related CAS # |
142825-10-3 (R-isomer);155320-20-0 (S-isomer);4478-93-7 (racemic);
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| SMILES |
O=S(CCCCN=C=S)C
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| InChi Key |
SUVMJBTUFCVSAD-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C6H11NOS2/c1-10(8)5-3-2-4-7-6-9/h2-5H2,1H3
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| Chemical Name |
1-isothiocyanato-4-(methylsulfinyl)butane
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| Synonyms |
Sulforafan Sulforaphane BroccoPhane Detoxophane Broccoli sprout extracts 4-methyl-sulfinybutyl isothiocyanatel
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 62.5 mg/mL (~352.53 mM)
H2O : ~50 mg/mL (~282.02 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (14.10 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (14.10 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (14.10 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 10 mg/mL (56.40 mM) in 30 % SBE-β-CD (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.6402 mL | 28.2008 mL | 56.4016 mL | |
| 5 mM | 1.1280 mL | 5.6402 mL | 11.2803 mL | |
| 10 mM | 0.5640 mL | 2.8201 mL | 5.6402 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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