Size | Price | Stock | Qty |
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500mg |
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1g |
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10g |
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Other Sizes |
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ln Vitro |
Sulforhodamine B sodium salt (SRB) is frequently used in cytotoxicity assays, which measure cellular proteins to determine cell density, or as a membrane-impermeable analyte. Numerous screening assays have been carried out at low cost using the SRB assay to investigate cytotoxicity in cell-based investigations. The technique is based on the characteristics of SRB, which binds to proteins stoichiometrically in slightly acidic conditions and can be extracted in alkaline conditions; consequently, the quantity of bound dye can induce cell development. The four primary steps of the protocol are as follows: preparation for processing, processing for cell proliferation and selection, fixing of the cells and SRB staining, and measurements of absorbance. This assay can be used sensitively to test small high-potency molecules in adherent cells or chemotherapeutic drugs, but it is limited to manual or semi-automated screening. It is employed in the assessment of the effects of gene expression regulation (knockdown, upregulation) and the investigation of the effects of miRNA replacement on cell division [1].
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References |
[1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984
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Molecular Formula |
C27H29N2O7S2-.NA+
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Molecular Weight |
580.64816
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CAS # |
3520-42-1
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
O=S(C1=CC=C(C2=C3C=CC(N(CC)CC)=CC3=[O+]C4=C2C=CC(N(CC)CC)=C4)C(S(=O)([O-])=O)=C1)([O-])=O.[Na+]
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~62.5 mg/mL (~107.64 mM)
H2O : ~50 mg/mL (~86.11 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (86.11 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
 (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.7222 mL | 8.6110 mL | 17.2221 mL | |
5 mM | 0.3444 mL | 1.7222 mL | 3.4444 mL | |
10 mM | 0.1722 mL | 0.8611 mL | 1.7222 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.