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1mg |
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5mg |
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10mg |
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25mg |
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50mg |
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100mg |
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250mg |
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Other Sizes |
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Purity: ≥98%
T16Ainh-A01 is an aminophenylthiazole analog acting as a novel and potent transmembrane protein 16A (TMEM16A) inhibitor which inhibits TMEM16A-mediated chloride currents with an IC50 value of ~1 µM. TMEM16A (ANO1) functions as a calcium-activated chloride channel (CaCC). TMEM16A inhibitors are potential development candidates for drug therapy of hypertension, pain, diarrhea, and excessive mucus production.
ln Vitro |
Without significantly affecting L-type Ca2+ current, T16Ainh-A01 (0.3-30 μM) greatly decreases the inward and outward components of IClCa and inhibits IClCa in RUICC [1]. At all voltages, TMEM16A chloride currents (caused by 275 nM free calcium in the pipette) are nearly entirely inhibited by T16Ainh-A01 (10 μM), suggesting a voltage-independent block mechanism [2].
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References |
[1]. Fedigan S, et al. Effects of new-generation TMEM16A inhibitors on calcium-activated chloride currents in rabbit urethral interstitial cells of Cajal. Pflugers Arch. 2017 Nov;469(11):1443-1455.
[2]. Namkung W, et al. TMEM16A inhibitors reveal TMEM16A as a minor component of calcium-activated chloride channel conductance in airway and intestinal epithelial cells. J Biol Chem. 2011 Jan 21;286(3):2365-74. |
Molecular Formula |
C19H20N4O3S2
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Molecular Weight |
416.5171
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Exact Mass |
416.1
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Elemental Analysis |
C, 54.79; H, 4.84; N, 13.45; O, 11.52; S, 15.39
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CAS # |
552309-42-9
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Appearance |
Solid powder
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SMILES |
O=C(NC1=NC(C2=CC=C(OC)C=C2)=CS1)CSC3=NC(C)=C(CC)C(N3)=O
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Synonyms |
T16Ainh-A01; T16Ainh A01; T16Ainh-A-01
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~83.33 mg/mL (~200.06 mM)
DMF :≥ 10 mg/mL (~24.01 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (4.99 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.4008 mL | 12.0042 mL | 24.0085 mL | |
5 mM | 0.4802 mL | 2.4008 mL | 4.8017 mL | |
10 mM | 0.2401 mL | 1.2004 mL | 2.4008 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.