Size | Price | Stock | Qty |
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50mg |
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100mg |
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Other Sizes |
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ln Vitro |
The cytotoxic cells exhibited action against HL60 leukemia when treated with tetrahydroxyquinone (100-500 μM) for 24 hours. The activity was assessed by total protein content (IC50 of 20 µM), phosphatase activity (IC50 of 40 µM), or MTT assay (IC50 of 45 µM). In HL60 leukemia cells, tetrahydroxyquinone is a strong inducer of ROS generation [1]. At concentrations higher than 25 µM, tetrahydroxyquinone efficiently activates caspase 3, which in turn promotes DNA fragmentation and phosphatidylserine exposure at the same concentration [1]. At as low as 25 µM, tetrahydroxyquinone causes the release of cytochrome c from mitochondria. Increased phosphorylation of Ser473 in protein kinase B (the Bad kinase of Ser112) is another effect of tetrahydroxyquinone therapy [1].
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Cell Assay |
Cell viability assay [1]
Cell Types: HL60 leukemia cells Tested Concentrations: 100 μM, 200 μM, 300 μM, 400 μM, 500 μM Incubation Duration: 24 hrs (hours) Experimental Results: Cytotoxic to HL60 leukemia cells. |
References |
[1]. Alexandre D Martins Cavagis, et al. Tetrahydroxyquinone induces apoptosis of leukemia cells through diminished survival signaling. Exp Hematol. 2006 Feb;34(2):188-96.
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Molecular Formula |
C6H4O6
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Molecular Weight |
172.09
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Exact Mass |
172.0
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CAS # |
319-89-1
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Related CAS # |
Tetrahydroxyquinone monohydrate;1215458-51-7
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
O=C1C(O)=C(O)C(C(O)=C1O)=O
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Synonyms |
Tetroquinonum; Tetroquinona; Tetroquinone
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~100 mg/mL (~581.09 mM)
H2O : ~1 mg/mL (~5.81 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (14.53 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (14.53 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 5.8109 mL | 29.0546 mL | 58.1091 mL | |
5 mM | 1.1622 mL | 5.8109 mL | 11.6218 mL | |
10 mM | 0.5811 mL | 2.9055 mL | 5.8109 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.